液相色谱-质谱联用代谢组学中的质量控制与验证问题

Quality Control and Validation Issues in LC-MS Metabolomics.

作者信息

Begou Olga, Gika Helen G, Theodoridis Georgios A, Wilson Ian D

机构信息

Department of Chemistry, Aristotle University, Thessaloniki, Greece.

School of Medicine, Aristotle University, Thessaloniki, Greece.

出版信息

Methods Mol Biol. 2018;1738:15-26. doi: 10.1007/978-1-4939-7643-0_2.

DOI:10.1007/978-1-4939-7643-0_2

PMID:29654580

Abstract

Global metabolic profiling (untargeted metabolomics) of different and complex biological matrices aims to implement an holistic, hypothesis-free analysis of (potentially) all the metabolites present in the analyzed sample. However, such an approach, although it has been the focus of great interest over the past few years, still faces many limitations and challenges, particularly with regard to the validation and the quality of the obtained results. The present protocol describes a quality control (QC) procedure for monitoring the precision of the analytical process involving untargeted metabolic phenotyping of urine and plasma/serum. The described/suggested methodology can be applied to different biological matrices, such as biological biofluids, cell, and tissue extracts.

摘要

对不同且复杂的生物基质进行全局代谢谱分析（非靶向代谢组学）旨在对分析样本中（潜在的）所有代谢物进行全面的、无假设分析。然而，尽管这种方法在过去几年一直备受关注，但仍面临许多限制和挑战，特别是在结果的验证和质量方面。本方案描述了一种质量控制（QC）程序，用于监测涉及尿液和血浆/血清非靶向代谢表型分析的分析过程的精密度。所描述/建议的方法可应用于不同的生物基质，如生物体液、细胞和组织提取物。