Wakayama Masataka, Hirayama Akiyoshi, Soga Tomoyoshi
Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
Methods Mol Biol. 2015;1277:113-22. doi: 10.1007/978-1-4939-2377-9_9.
Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.
毛细管电泳-质谱联用（CE-MS）已被证明在代谢组学研究中很有用。带电代谢物首先通过CE根据电荷和大小进行分离，随后使用MS进行选择性检测。CE-MS的主要优点是其高分辨率以及几乎任何带电物质都可以通过阳离子和阴离子两种方法进行分析这一事实。该技术可以很容易地应用于源自细菌、植物、哺乳动物和体液的各种类型的生物样品。本章重点介绍了使用该技术的详细实际操作程序。
