Barnes Stephen, Benton H Paul, Casazza Krista, Cooper Sara J, Cui Xiangqin, Du Xiuxia, Engler Jeffrey, Kabarowski Janusz H, Li Shuzhao, Pathmasiri Wimal, Prasain Jeevan K, Renfrow Matthew B, Tiwari Hemant K
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
Department of Pharmacology and Toxicology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
J Mass Spectrom. 2016 Jul;51(7):461-75. doi: 10.1002/jms.3782.
The study of metabolism has had a long history. Metabolomics, a systems biology discipline representing analysis of known and unknown pathways of metabolism, has grown tremendously over the past 20 years. Because of its comprehensive nature, metabolomics requires careful consideration of the question(s) being asked, the scale needed to answer the question(s), collection and storage of the sample specimens, methods for extraction of the metabolites from biological matrices, the analytical method(s) to be employed and the quality control of the analyses, how collected data are correlated, the statistical methods to determine metabolites undergoing significant change, putative identification of metabolites and the use of stable isotopes to aid in verifying metabolite identity and establishing pathway connections and fluxes. The National Institutes of Health Common Fund Metabolomics Program was established in 2012 to stimulate interest in the approaches and technologies of metabolomics. To deliver one of the program's goals, the University of Alabama at Birmingham has hosted an annual 4-day short course in metabolomics for faculty, postdoctoral fellows and graduate students from national and international institutions. This paper is the first part of a summary of the training materials presented in the course to be used as a resource for all those embarking on metabolomics research. The complete set of training materials including slide sets and videos can be viewed at http://www.uab.edu/proteomics/metabolomics/workshop/workshop_june_2015.php. Copyright © 2016 John Wiley & Sons, Ltd.
代谢研究有着悠久的历史。代谢组学作为一门系统生物学学科，代表了对已知和未知代谢途径的分析，在过去20年里得到了极大的发展。由于其综合性，代谢组学需要仔细考虑所提出的问题、回答这些问题所需的规模、样本标本的收集和储存、从生物基质中提取代谢物的方法、所采用的分析方法以及分析的质量控制、如何关联所收集的数据、确定发生显著变化的代谢物的统计方法、代谢物的推定鉴定以及使用稳定同位素来帮助验证代谢物身份并建立途径连接和通量。美国国立卫生研究院共同基金代谢组学计划于2012年设立，以激发对代谢组学方法和技术的兴趣。为实现该计划的一个目标，阿拉巴马大学伯明翰分校为来自国内和国际机构的教师、博士后和研究生举办了为期4天的年度代谢组学短期课程。本文是该课程所展示的培训材料总结的第一部分，可供所有从事代谢组学研究的人员作为参考资源。完整的培训材料集，包括幻灯片集和视频，可在http://www.uab.edu/proteomics/metabolomics/workshop/workshop_june_2015.php查看。版权所有© 2016约翰威立父子有限公司。
