Ming Jinfa, Pan Fukui, Zuo Baoqi
The College of Textiles & Fashion, Qingdao University, Qingdao 266071, China; National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
The College of Textiles & Fashion, Qingdao University, Qingdao 266071, China.
Int J Biol Macromol. 2015 Apr;75:398-401. doi: 10.1016/j.ijbiomac.2015.02.002. Epub 2015 Feb 9.
Regenerated silk fibroin aqueous solution was used to study the crystalline structure of Bombyx mori silk fibroin in vitro. By controlling environmental conditions and concentration of silk fibroin solution, it provided a means for the direct preparing silk I structure and understanding the details of silk fibroin molecules interactions in formation process. In this study, silk fibroin molecules were assembled to form random coil at low concentration of solution and then, as the concentration increases, were converted to silk I at 55% relative humidity (RH). At the same time, the structure of silk fibroin forming below 45 °C was mostly in silk I. A partial ternary phase diagram of temperature-humidity-concentration was constructed based on the results. The results showed silk I structure could be controlled by adjusting the external environmental conditions. The enhanced control over silk I structure, as embodied in phase diagram, could potentially be utilized to understand the molecular chain conformation of silk I in further research work.
再生丝素蛋白水溶液用于体外研究家蚕丝素蛋白的晶体结构。通过控制环境条件和丝素蛋白溶液浓度，它提供了一种直接制备丝I结构并了解丝素蛋白分子在形成过程中相互作用细节的方法。在本研究中，丝素蛋白分子在低溶液浓度下组装形成无规卷曲，然后随着浓度增加，在相对湿度（RH）为55%时转变为丝I。同时，在45℃以下形成的丝素蛋白结构大多为丝I。基于这些结果构建了温度-湿度-浓度的部分三元相图。结果表明，通过调节外部环境条件可以控制丝I结构。相图中体现的对丝I结构的增强控制，有可能在进一步的研究工作中用于理解丝I的分子链构象。
