用夹心酶联免疫吸附测定法检测实验感染巨片形吸虫的家兔粪便抗原

Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica.

作者信息

Shalaby Said Ibrahim, El-Bahy Mohammad, Shalaby Samy Ibrahim Aboulella, Shalaby Hatem, Gupta Neelima, Gupta Dileep Kumar

机构信息

Department of Complementary Medicine, Medical Division, National Research Centre, Cairo, Egypt.

Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.

出版信息

Iran J Parasitol. 2014 Sep;9(3):374-81.

PMID:25678922

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4316569/

Abstract

BACKGROUND

The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits.

METHODS

Copro Hyper Immune Serum (HIS) and Excretory-Secretory Hyper Immune Serum (ES HIS) antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infected with different doses of F. gigantica encysted metacercariae (EMC) (10, 25 and 30 EMC). The relation between time of appearance of coproantigens in feces and anti-Fasciola antibodies in serum was evaluated.

RESULTS

The earliest diagnostic coproantigen was recorded at 21(st), 25(th) and 28(th) day post-infection (p.i.) in groups of rabbits infected with 30, 25 and 10 F. gigantica EMC respectively. Both HIS and ES HIS were able to detect coproantigens in feces of rabbits infected with 30 EMC at day 21 p.i. The appearance of F. gigantica coproantigens in feces of infected rabbits was concurrent to the appearance of anti-Fasciola antibodies in blood (3(rd) week p.i.). However, coproantigen has specific ability for direct assessment of active infection with minimal cross-reaction with other heterologous parasitic infections.

CONCLUSION

The findings hold promise for a more accurate diagnostic technique in the near future for suspected Fasciola infection.

摘要

背景

本研究旨在报告在实验感染巨片形吸虫的家兔粪便中粪抗原的出现情况以及血清中循环抗体的情况。

方法

使用粪超免疫血清（HIS）和排泄分泌超免疫血清（ES HIS）抗原，通过夹心酶联免疫吸附测定法（ELISA）检测12只实验感染不同剂量（10、25和30个）巨片形吸虫囊蚴（EMC）的家兔粪便中的巨片形吸虫抗原。评估粪便中粪抗原出现时间与血清中抗片形吸虫抗体之间的关系。

结果

分别在感染30、25和10个巨片形吸虫EMC的家兔组中，最早在感染后第21、25和28天记录到诊断性粪抗原。HIS和ES HIS均能够在感染后第21天检测到感染30个EMC的家兔粪便中的粪抗原。感染家兔粪便中巨片形吸虫粪抗原的出现与血液中抗片形吸虫抗体的出现同时发生（感染后第3周）。然而，粪抗原具有直接评估活动性感染的特定能力，与其他异源寄生虫感染的交叉反应最小。