Rodríguez-Pérez J, Hillyer G V
Department of Pathology and Laboratory Medicine, University of Puerto Rico School of Medicine, San Juan 00936-5067, USA.
Vet Parasitol. 1995 Jan;56(1-3):57-66. doi: 10.1016/0304-4017(94)00666-z.
Two groups of sheep were exposed to Fasciola hepatica and Schistosoma mansoni/F. hepatica infections, respectively, to determine the time of appearance of F. hepatica circulating excretory-secretory (FhES) antigens. Five sheep were infected orally with 400 F. hepatica metacercariae. Five additional sheep were infected first percutaneously with 5000 S. mansoni cercariae, and 10 weeks later orally with 400 F. hepatica metacercariae. Antigen detection was performed in the two groups by a double antibody microELISA. In the group infected with F. hepatica, circulating FhES antigens were detected in all five animals. In the group infected first with S. mansoni none of the sera had positive optical density values for FhES antigens throughout the 10 week period following infection. After F. hepatica challenge, FhES antigens were detected in all animals. Thus, sheep infected with S. mansoni did not develop detectable F. hepatica circulating antigens during the 10 week period following Schistosoma infection. Anti-FhES antibodies were detected by FAST-ELISA, and all animals infected with F. hepatica were positive. In the group infected with S. mansoni, cross-reactive antibodies to F. hepatica were detected by 4-6 weeks post infection. All sheep with double infections showed high antibody levels by 2 weeks after infection with F. hepatica.
将两组绵羊分别暴露于肝片吸虫以及曼氏血吸虫/肝片吸虫感染中，以确定肝片吸虫循环排泄-分泌（FhES）抗原出现的时间。五只绵羊经口感染400个肝片吸虫囊蚴。另外五只绵羊先经皮感染5000个曼氏血吸虫尾蚴，10周后经口感染400个肝片吸虫囊蚴。通过双抗体微ELISA对两组进行抗原检测。在感染肝片吸虫的组中，所有五只动物均检测到循环FhES抗原。在首先感染曼氏血吸虫的组中，在感染后的10周内，所有血清的FhES抗原光密度值均为阴性。在受到肝片吸虫攻击后，所有动物均检测到FhES抗原。因此，感染曼氏血吸虫的绵羊在感染血吸虫后的10周内未产生可检测到的肝片吸虫循环抗原。通过FAST-ELISA检测抗FhES抗体，所有感染肝片吸虫的动物均为阳性。在感染曼氏血吸虫的组中，感染后4-6周检测到针对肝片吸虫的交叉反应性抗体。所有双重感染的绵羊在感染肝片吸虫后2周时均显示出高抗体水平。
