Kouris Anargyros, Pistiki Aikaterini, Katoulis Alexandros, Georgitsi Marianna, Giatrakou Sofia, Papadavid Evangelia, Netea Mihai G, Stavrianeas Nikolaos, Giamarellos-Bourboulis Evangelos J
2nd Department of Dermatology and Venereology, National and Kapodistrian University of Athens, Medical School, Athens, Greece.
4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, Athens, Greece.
Eur Cytokine Netw. 2014 Oct-Dec;25(4):63-8. doi: 10.1684/ecn.2014.0358.
Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases. Proinflammatory cytokines play an important pathogenetic role at a local level.
To assess whether the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis.
Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes involved and the intracellular TNF-α production in monocytes.
IL-17 levels were significantly higher in the supernatants of PBMCs from psoriatic patients after stimulation with phytohemagglutinin. TNF-α production was also significantly higher in cells from psoriatic patients after stimulation with all stimuli, as compared with health volunteers. Similar changes were not found for the other cytokines. A statistically significant difference was observed between patients and controls for inflammatory CD14(+)/CD16(+) monocytes (p<0.0001) and patrolling CD14-/CD16(+) monocytes.
Hyper-production of TNF-α is documented in psoriasis. These results support the concept that there is a systemic, proinflammatory component in psoriasis.
银屑病是最常见的免疫介导慢性炎症性皮肤病之一。促炎细胞因子在局部水平发挥重要的致病作用。
评估银屑病患者体内促炎细胞因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-17（IL-17）、白细胞介素-22（IL-22）和肿瘤坏死因子-α（TNF-α）是否会全身性释放。
从30例银屑病患者和30名健康志愿者中分离外周血单个核细胞（PBMCs）。用微生物刺激物刺激PBMCs后，采用酶免疫测定法评估上清液中细胞因子的产生情况。此外，采用流式细胞术确定参与的单核细胞亚群以及单核细胞内TNF-α的产生情况。
用植物血凝素刺激后，银屑病患者PBMCs上清液中的IL-17水平显著更高。与健康志愿者相比，用所有刺激物刺激后，银屑病患者细胞中的TNF-α产生也显著更高。其他细胞因子未发现类似变化。炎症性CD14(+)/CD16(+)单核细胞（p<0.0001）和巡逻性CD14-/CD16(+)单核细胞在患者和对照组之间观察到统计学上的显著差异。
银屑病中存在TNF-α的过度产生。这些结果支持银屑病存在全身性促炎成分这一概念。
