Bustamante Verónica, Meza Paulina, Román Juan C, García Patricia
Rev Chilena Infectol. 2014 Dec;31(6):670-5. doi: 10.4067/S0716-10182014000600005.
Automated systems have simplified laboratory workflow, improved standardization, traceability and diminished human errors and workload. Although microbiology laboratories have little automation, in recent years new tools for automating pre analytical steps have appeared.
To assess the performance of an automated streaking machine for urine cultures and its agreement with the conventional manual plating method for semi quantitative colony counts.
495 urine samples for urinary culture were inoculated in CPS® agar using our standard protocol and the PREVI™ Isola. Rates of positivity, negativity, polymicrobial growth, bacterial species, colony counts and re-isolation requirements were compared.
Agreement was achieved in 98.97% of the positive/negative results, in 99.39% of the polymicrobial growth, 99.76% of bacterial species isolated and in 98.56 % of colony counts. The need for re-isolation of colonies decreased from 12.1% to 1.1% using the automated system.
PREVI™ Isola's performance was as expected, time saving and improving bacterial isolation. It represents a helpful tool for laboratory automation.
自动化系统简化了实验室工作流程，提高了标准化、可追溯性，减少了人为误差和工作量。尽管微生物实验室的自动化程度较低，但近年来出现了用于自动化分析前步骤的新工具。
评估一种用于尿液培养的自动划线机的性能及其与传统手动平板接种法在半定量菌落计数方面的一致性。
按照我们的标准方案，使用PREVI™ Isola在CPS®琼脂中接种495份用于尿液培养的尿液样本。比较阳性、阴性、多种微生物生长、细菌种类、菌落计数和重新分离要求的发生率。
在98.97%的阳性/阴性结果、99.39%的多种微生物生长、99.76%的分离细菌种类和98.56%的菌落计数方面达成了一致。使用自动化系统，菌落重新分离的需求从12.1%降至1.1%。
PREVI™ Isola的性能符合预期，节省时间并改善了细菌分离。它是实验室自动化的一个有用工具。
