Hartman Tiffiney R, Ventresca Erin M, Hopkins Anthony, Zinshteyn Daniel, Singh Tanu, O'Brien Jenny A, Neubert Benjamin C, Hartman Matthew G, Schofield Heather K, Stavrides Kevin P, Talbot Danielle E, Riggs Devon J, Pritchard Caroline, O'Reilly Alana M
Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Molecular Cell Biology and Genetics Graduate Program, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
Genetics. 2015 Apr;199(4):935-57. doi: 10.1534/genetics.114.173617. Epub 2015 Feb 12.
In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the Drosophila ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.
在许多组织中，干细胞的存在是通过组织维持体内平衡以及在损伤后进行修复的能力推断出来的。分离出具有在特定组织内产生全套细胞能力的自我更新细胞有力地支持了这一观点，但在其微环境中对单个干细胞进行识别和基因操作仍然是一项挑战。在此，我们展示了用于标记和基因改造果蝇卵巢内上皮卵泡干细胞（FSC）的新方法。利用这些新工具，我们定义了一个连续的多步骤过程，该过程包括FSC从静止状态转变为增殖状态。我们进一步证明，整合素在FSC内是细胞自主所需的，以提供该过程每个步骤所必需的定向信号。这些方法可用于确定特定基因在静止一段时间后FSC分裂过程中发生的连续事件中的精确作用。
