Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
STAR Protoc. 2021 Jun 11;2(2):100592. doi: 10.1016/j.xpro.2021.100592. eCollection 2021 Jun 18.
We have outlined the approach of visualizing autophagy specifically in the epithelial follicle stem cells of the ovary using the LysoTracker dye. The advantage of using this protocol is that it details several techniques, including ovary dissection, immunofluorescence, and western blotting, that positively identify autophagy changes in a very small population of cells. One of the limitations of this protocol is that it needs to be combined with other genetic manipulations and positive markers of the autophagy pathway. For complete details on the use and execution of this protocol, please refer to Singh et al., (2018).
我们使用 LysoTracker 染料概述了在卵巢上皮滤泡干细胞中特异性可视化自噬的方法。使用此方案的优点在于，它详细介绍了几种技术，包括卵巢解剖、免疫荧光和 Western blot，可明确鉴定非常小的细胞群体中的自噬变化。该方案的局限性之一是需要与其他遗传操作和自噬途径的阳性标记物结合使用。有关使用和执行此方案的完整详细信息，请参阅 Singh 等人，（2018 年）。
