Nakagawa Y, Muneta T, Kondo S, Mizuno M, Takakuda K, Ichinose S, Tabuchi T, Koga H, Tsuji K, Sekiya I
Department of Joint Surgery and Sports Medicine, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Osteoarthritis Cartilage. 2015 Jun;23(6):1007-17. doi: 10.1016/j.joca.2015.02.008. Epub 2015 Feb 13.
The induction of synovial tissue to the meniscal lesion is crucial for meniscal healing. Synovial Mesenchymal stem cells (MSCs) are an attractive cell source because of their high proliferative and chondrogenic potentials. We examined whether transplantation of synovial MSCs promoted healing after meniscal repair of extended longitudinal tear of avascular area in a microminipig model.
Longitudinal tear lesion was made in medial menisci and sutured in both knees, and then a synovial MSC suspension was administered for 10 min only in unilateral knee. The sutured meniscus was evaluated morphologically and biomechanically at 2, 4, and 12 weeks. The behavior of transplanted MSCs was also examined.
The meniscal healing at 12 weeks was significantly better in the MSC group than in the control group; macroscopically, histologically and by T1rho mapping analysis. Transmission electron microscopic analysis demonstrated that the meniscus lesion was occupied by dense collagen fibrils only in the MSC group. Biomechanical analysis revealed that the tensile strength to failure of the meniscus higher in the MSC group than in the control group in each microminipig. Synovial tissue covered better along the superficial layer from the outer zone into the lesion of the meniscus in the MSC group at 2 and 4 weeks in each microminipig. Synovial MSCs labeled with ferucarbotran were detected in the meniscus lesion and adjacent synovium by MRI at 2 weeks.
Transplantation of synovial MSCs promoted healing after meniscal repair with induction of synovium into the longitudinal tear in the avascular zone of meniscus in pigs.
诱导滑膜组织至半月板损伤处对半月板愈合至关重要。滑膜间充质干细胞（MSCs）因其高增殖和软骨形成潜能而成为有吸引力的细胞来源。我们在微型猪模型中研究了滑膜间充质干细胞移植是否能促进无血管区延长纵向撕裂的半月板修复后的愈合。
在内侧半月板制造纵向撕裂损伤并在双膝关节进行缝合，然后仅在单侧膝关节给予滑膜间充质干细胞悬液10分钟。在2周、4周和12周时对缝合的半月板进行形态学和生物力学评估。还检查了移植的间充质干细胞的行为。
在12周时，间充质干细胞组的半月板愈合在宏观、组织学和T1rho成像分析方面均明显优于对照组。透射电子显微镜分析表明，仅在间充质干细胞组中半月板损伤处被致密的胶原纤维占据。生物力学分析显示，在每只微型猪中，间充质干细胞组半月板的拉伸强度高于对照组。在每只微型猪的2周和4周时，间充质干细胞组的滑膜组织从外侧区沿表层更好地覆盖至半月板损伤处。在2周时通过MRI在半月板损伤处和相邻滑膜中检测到用超顺磁性氧化铁标记的滑膜间充质干细胞。
滑膜间充质干细胞移植通过诱导滑膜进入猪半月板无血管区的纵向撕裂处，促进了半月板修复后的愈合。
