Özcan Hakkı Mevlüt, Sezgintürk Mustafa Kemal
Chemistry Dept., Faculty of Science, University of Trakya, Edirne, Turkey.
Chemistry Dept., Faculty of Arts and Sciences, Namık Kemal University, Tekirdağ, Turkey.
Biotechnol Prog. 2015 May-Jun;31(3):815-22. doi: 10.1002/btpr.2060. Epub 2015 Feb 26.
This paper presents a novel hormone-based impedimetric biosensor to determine parathyroid hormone (PTH) level in serum for diagnosis and monitoring treatment of hyperparathyroidism, hypoparathyroidism and thyroid cancer. The interaction between PTH and the biosensor was investigated by an electrochemical method. The biosensor was based on the gold electrode modified by 12-mercapto dodecanoic (12MDDA). Antiparathyroid hormone (anti-PTH) was covalently immobilized on to poly amidoamine dendrimer (PAMAM) which was bound to a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) couple, self-assembled monolayer structure from one of the other NH2 sites. The immobilization of anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscope techniques. After the optimization studies of immobilization materials such as 12MDDA, EDC-NHS, PAMAM, and glutaraldehyde, the performance of the biosensor was investigated in terms of linearity, sensitivity, repeatability, and reproducibility. PTH was detected within a linear range of 10-60 fg/mL. Finally the described biosensor was used to monitor PTH levels in artificial serum samples.
本文提出了一种新型的基于激素的阻抗式生物传感器，用于测定血清中的甲状旁腺激素（PTH）水平，以诊断和监测甲状旁腺功能亢进、甲状旁腺功能减退和甲状腺癌的治疗情况。通过电化学方法研究了PTH与生物传感器之间的相互作用。该生物传感器基于由12-巯基十二烷酸（12MDDA）修饰的金电极。抗甲状旁腺激素（anti-PTH）共价固定在聚酰胺胺树枝状大分子（PAMAM）上，PAMAM与1-乙基-3-（3-二甲基氨基丙基）-碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）偶联物结合，从另一个NH2位点自组装成单层结构。通过电化学阻抗谱、循环伏安法和扫描电子显微镜技术监测anti-PTH的固定情况。在对12MDDA、EDC-NHS、PAMAM和戊二醛等固定材料进行优化研究后，从线性、灵敏度、重复性和再现性方面研究了生物传感器的性能。在10-60 fg/mL的线性范围内检测到了PTH。最后，将所描述的生物传感器用于监测人工血清样品中的PTH水平。
