Shi Zhenhua, Yu Hui, Sun Yongyan, Yang Chuanjun, Lian Huiyong, Cai Peng
1] Physical Environment Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, P. R. China [2] University of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, P. R. China.
Physical Environment Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, P. R. China.
Sci Rep. 2015 Feb 16;5:8471. doi: 10.1038/srep08471.
A literal mountain of documentation generated in the past five decades showing unmistakable health hazards associated with extremely low-frequency electromagnetic fields (ELF-EMFs) exposure. However, the relation between energy mechanism and ELF-EMF exposure is poorly understood. In this study, Caenorhabditis elegans was exposed to 50 Hz ELF-EMF at intensities of 0.5, 1, 2, and 3 mT, respectively. Their metabolite variations were analyzed by GC-TOF/MS-based metabolomics. Although minimal metabolic variations and no regular pattern were observed, the contents of energy metabolism-related metabolites such as pyruvic acid, fumaric acid, and L-malic acid were elevated in all the treatments. The expressions of nineteen related genes that encode glycolytic enzymes were analyzed by using quantitative real-time PCR. Only genes encoding GAPDH were significantly upregulated (P < 0.01), and this result was further confirmed by western blot analysis. The enzyme activity of GAPDH was increased (P < 0.01), whereas the total intracellular ATP level was decreased. While no significant difference in lifespan, hatching rate and reproduction, worms exposed to ELF-EMF exhibited less food consumption compared with that of the control (P < 0.01). In conclusion, C. elegans exposed to ELF-EMF have enhanced energy metabolism and restricted dietary, which might contribute to the resistance against exogenous ELF-EMF stress.
在过去的五十年里产生了如山般的文献资料，显示出与极低频电磁场（ELF-EMFs）暴露相关的明确健康危害。然而，能量机制与ELF-EMF暴露之间的关系却鲜为人知。在本研究中，秀丽隐杆线虫分别暴露于强度为0.5、1、2和3 mT的50 Hz ELF-EMF中。通过基于气相色谱-飞行时间质谱（GC-TOF/MS）的代谢组学分析它们的代谢物变化。尽管观察到最小的代谢变化且没有规律模式，但在所有处理中，与能量代谢相关的代谢物如丙酮酸、富马酸和L-苹果酸的含量均有所升高。使用定量实时PCR分析了十九个编码糖酵解酶的相关基因的表达。只有编码甘油醛-3-磷酸脱氢酶（GAPDH）的基因显著上调（P < 0.01），并且蛋白质免疫印迹分析进一步证实了这一结果。GAPDH的酶活性增加（P < 0.01），而细胞内总ATP水平降低。虽然在寿命、孵化率和繁殖方面没有显著差异，但与对照组相比，暴露于ELF-EMF的线虫食物消耗量减少（P < 0.01）。总之，暴露于ELF-EMF的秀丽隐杆线虫能量代谢增强且饮食受限，这可能有助于抵抗外源性ELF-EMF应激。
