Hritcu Lucian, Stefan Marius, Brandsch Roderich, Mihasan Marius
Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania.
Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania.
Neurosci Lett. 2015 Mar 30;591:41-47. doi: 10.1016/j.neulet.2015.02.014. Epub 2015 Feb 13.
6-Hydroxy-l-nicotine (6HLN) is a nicotine metabolite resulted from nicotine degradation within Arthrobacter nicotinovorans with positive effects on spatial memory and oxidative stress damage. In the present study, the effects of 6HLN on spatial memory performance were assessed in scopolamine-treated rats. Scopolamine-induced memory impairments were observed, as measured by the Y-maze and radial arm-maze tasks. Decreased activities of superoxide dismutase, glutathione peroxidase and catalase along with decrease of total content of reduced glutathione were observed in the rat hippocampal homogenates of scopolamine-treated animals as compared with control. Production of malondialdehyde (lipid peroxidation) significantly increased in the rat hippocampal homogenates of scopolamine-treated animals as compared with control, as a consequence of impaired antioxidant enzymes activities. Additionally, in scopolamine-treated rats 6HLN significantly improved memory formation and decreased oxidative stress, suggesting memory-enhancing and antioxidant effects. Therefore, our results suggest that administration of 6HLN ameliorates scopolamine-induced spatial memory impairment by attenuation of the oxidative stress in the rat hippocampus.
6-羟基-1-尼古丁（6HLN）是节杆菌中尼古丁降解产生的一种尼古丁代谢产物，对空间记忆和氧化应激损伤具有积极作用。在本研究中，评估了6HLN对东莨菪碱处理大鼠空间记忆表现的影响。通过Y迷宫和放射状臂迷宫任务测量，观察到东莨菪碱诱导的记忆损伤。与对照组相比，东莨菪碱处理动物的大鼠海马匀浆中超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性降低，同时还原型谷胱甘肽的总含量也降低。与对照组相比，东莨菪碱处理动物的大鼠海马匀浆中丙二醛（脂质过氧化）的产生显著增加，这是抗氧化酶活性受损的结果。此外，在东莨菪碱处理的大鼠中，6HLN显著改善了记忆形成并降低了氧化应激，表明具有增强记忆和抗氧化作用。因此，我们的结果表明，给予6HLN可通过减轻大鼠海马中的氧化应激来改善东莨菪碱诱导的空间记忆损伤。
