西红花苷对溴化乙锭诱导大鼠脑脱髓鞘后某些应激氧化标志物的调节作用

Moderating effects of crocin on some stress oxidative markers in rat brain following demyelination with ethidium bromide.

作者信息

Fathimoghadam Hadi, Farbod Yaghoub, Ghadiri Ataallah, Fatemi Rouholah

机构信息

Physiology Research Center (PRC), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Heliyon. 2019 Feb 15;5(2):e01213. doi: 10.1016/j.heliyon.2019.e01213. eCollection 2019 Feb.

BACKGROUND

The purpose of this study was to investigate the effects of Crocin on oxidative markers (GPx, SOD, MDA) in animal model of demyelination with Ethidium bromide (EB).

METHODS

Female Wistar rats were assigned in to 4 groups; Sham, with no receiving any agent (Sham), Sham Operated group with injection of EB into the brain received no agent (SO), Sham Treatment group with injection of EB and receiving PBS as vehicle and Treatment group with injection of EB and receiving Crocin (100 mg/kg). Demyelination was induced by single dose injection of 10 μl of EB 0.1% into the Cisterna magna of the brain. Crocin was diluted and applied to each animal for 21 days, once per day gavage. The levels of oxidative markers (GPx, SOD and MDA) were measured by related standard kits. Data were analyzed by paired t-test and ANOVA with post hoc test.

RESULTS

The results showed that crocin decreases the levels of GPx and SOD significantly as well as MDA level after 21 days (α ≤ 0.05). In addition, results showed that there were significant differences in the GPx, SOD and MDA levels between all groups at post treatment phase (α ≤ 0.05).

CONCLUSION

It can be concluded that crocin can moderate the level of oxidative markers after demyelination of the brain cells in MS cases. Due to this effect, crocin can be considered as an effective anti-oxidant in management of degenerative nervous system diseases.

背景

本研究旨在探讨西红花苷对溴化乙锭（EB）诱导的脱髓鞘动物模型中氧化标志物（谷胱甘肽过氧化物酶、超氧化物歧化酶、丙二醛）的影响。

方法

将雌性Wistar大鼠分为4组；假手术组，不接受任何药物（假手术组）；假手术操作组，向脑内注射EB但不接受任何药物（SO组）；假治疗组，注射EB并接受PBS作为载体；治疗组，注射EB并接受西红花苷（100 mg/kg）。通过向脑大池单次注射10 μl 0.1%的EB诱导脱髓鞘。将西红花苷稀释后每天经口灌胃给药每只动物，持续21天。使用相关标准试剂盒测量氧化标志物（谷胱甘肽过氧化物酶、超氧化物歧化酶和丙二醛）的水平。数据采用配对t检验和方差分析以及事后检验进行分析。