上皮-间质转化标志物E-钙黏蛋白和Slug在喉鳞状细胞癌中的预后作用。

The prognostic role of the epithelial-mesenchymal transition markers E-cadherin and Slug in laryngeal squamous cell carcinoma.

作者信息

Cappellesso Rocco, Marioni Gino, Crescenzi Marika, Giacomelli Luciano, Guzzardo Vincenza, Mussato Alessio, Staffieri Alberto, Martini Alessandro, Blandamura Stella, Fassina Ambrogio

机构信息

Surgical Pathology and Cytopathology Unit, Department of Medicine, University of Padova, Padova, Italy.

Otolaryngology Section, Department of Neurosciences, University of Padova, Padova, Italy.

出版信息

Histopathology. 2015 Oct;67(4):491-500. doi: 10.1111/his.12668. Epub 2015 Mar 31.

DOI:10.1111/his.12668

PMID:25684546

Abstract

AIMS

Laryngeal squamous cell carcinoma (LSCC) prognosis is definitely related to lymph node metastasis. Epithelial-mesenchymal transition (EMT) allows neoplastic cells to gain the plasticity and motility required for tumour progression and metastasis. The aim of this study was to investigate the role of EMT in the prognosis of LSCC.

METHODS AND RESULTS

Immunohistochemical analysis of E-cadherin, N-cadherin, Snail, Slug, ZEB1, and ZEB2 was performed in 37 consecutive LSCC cases. Low E-cadherin levels and high Slug levels correlated with both disease recurrence (P = 0.02 and P =0.01, respectively) and shorter disease-free survival (DFS) (P = 0.04 and P = 0.02, respectively). Relative expression levels of CDH1, SNAI2, miR-1 and the miR-200 family were also evaluated. CDH1, miR-200a and miR-200c down-regulation and SNAI2 overexpression were significantly associated with disease recurrence (P = 0.03, P = 0.02, P = 0.04, and P = 0.04, respectively).

CONCLUSIONS

EMT increases tumour recurrence risk and shortens DFS in LSCC. E-cadherin and Slug immunohistochemical analysis could be useful for identifying patients requiring more aggressive treatment after surgery.

摘要

目的

喉鳞状细胞癌（LSCC）的预后与淋巴结转移密切相关。上皮-间质转化（EMT）使肿瘤细胞获得肿瘤进展和转移所需的可塑性和运动性。本研究旨在探讨EMT在LSCC预后中的作用。

方法与结果

对37例连续的LSCC病例进行E-钙黏蛋白、N-钙黏蛋白、Snail、Slug、ZEB1和ZEB2的免疫组织化学分析。E-钙黏蛋白水平低和Slug水平高与疾病复发（分别为P = 0.02和P = 0.01）及无病生存期（DFS）较短（分别为P = 0.04和P = 0.02）均相关。还评估了CDH1、SNAI2、miR-1和miR-200家族的相对表达水平。CDH1、miR-200a和miR-200c下调以及SNAI2过表达与疾病复发显著相关（分别为P = 0.03、P = 0.02、P = 0.04和P = 0.04）。