Snyman Leendert D, Flett Bradley C, Schultz Rowina A
Department of Toxicology, Agricultural Research Council, Onderstepoort Veterinary Institute.
Onderstepoort J Vet Res. 2014 Nov 12;81(1):e1-e4. doi: 10.4102/ojvr.v81i1.765.
Diplonine, a mycotoxin that induces neurotoxic clinical signs in the guinea pig, resembling those occurring in cattle and sheep with diplodiosis, was isolated previously from a Stenocarpella maydisculture. Knowledge of the chemical properties of the toxin, which was characterised as a substituted ß-cyclopropylamino acid, enabled amendments in the present study to the initial steps of the isolation procedure. Extraction with water and fractionation by cation exchange chromatography improved the efficiency of isolation, potentially allowing the preparation of larger amounts of the toxin.
双孢霉素是一种能在豚鼠中诱发神经毒性临床症状的霉菌毒素，类似于患有双孢霉病的牛和羊所出现的症状，此前已从玉米小斑病菌培养物中分离得到。该毒素被鉴定为一种取代的β-环丙基氨基酸，对其化学性质的了解使得本研究对分离程序的初始步骤进行了改进。用水提取并通过阳离子交换色谱法进行分馏提高了分离效率，有可能制备出更多量的毒素。
