Masango Mxolisi G, Ferreira Gezina C H, Ellis Charlotte E, Elgorashi Esameldin E, Botha Christo J
Toxicology and Ethnoveterinary Medicine, Food, Feed and Veterinary Public Health (FFVPH) Programme, Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI), P/Bag x05, Onderstepoort, 0110, South Africa; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, P/Bag x04, Onderstepoort, 0110, South Africa.
Toxicology and Ethnoveterinary Medicine, Food, Feed and Veterinary Public Health (FFVPH) Programme, Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI), P/Bag x05, Onderstepoort, 0110, South Africa.
Toxicon. 2014 May;82:26-9. doi: 10.1016/j.toxicon.2014.02.002. Epub 2014 Feb 13.
The cytotoxicity of three Stenocarpella maydis metabolites (diplodiatoxin, dipmatol and diplonine) was investigated on Neuro-2a, CHO-K1 and MDBK cell lines. Diplodiatoxin was the most cytotoxic followed by dipmatol. Conversely, diplonine was not cytotoxic. Diplodiatoxin and dipmatol affected mitochondrial succinate dehydrogenase (MTT assay) and the overall viability of cells as assessed in real-time (xCELLigence assay). The results obtained so far indicate that diplodiatoxin and dipmatol exert their toxicity possibly via the necrotic cell death pathway.
研究了三种玉米小斑病菌代谢产物（双斑毒素、双斑醇和双斑宁）对Neuro-2a、CHO-K1和MDBK细胞系的细胞毒性。双斑毒素的细胞毒性最强，其次是双斑醇。相反，双斑宁没有细胞毒性。双斑毒素和双斑醇影响线粒体琥珀酸脱氢酶（MTT法）以及实时评估的细胞总体活力（xCELLigence法）。目前获得的结果表明，双斑毒素和双斑醇可能通过坏死性细胞死亡途径发挥其毒性。
