Li Hanwen, Gu Jisheng, Shah Luqman Ali, Siddiq Mohammad, Hu Jianhua, Cai Xiaobing, Yang Dong
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China.
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:210-216. doi: 10.1016/j.msec.2014.12.082. Epub 2014 Dec 30.
A novel bone cement pellet, with sustained release of vancomycin (VAN), was prepared by mixing VAN loaded mesoporous silica nanoparticle (MSN) and calcium sulfate α-hemihydrate (CS) together. To improve the VAN loading ability, MSN was functionalized with aminopropyltriethoxysilane (APS) to give APS-MSN. The VAN loading content and entrapment efficiency of APS-MSN could reach up to 45.91±0.81% and 84.88±1.52%, respectively, much higher than those of MSN, which were only 3.91% and 4.07%, respectively. The nitrogen adsorption-desorption measurement results demonstrated that most of the VAN were in the pores of APS-MSN. The CS/VAN@APS-MSN composite pellet showed a strongly drug sustained release effect in comparison with CS control pellet. The in vitro cell assays demonstrated that CS/APS-MSN composite was highly biocompatible and suitable to use as bone cement. Furthermore, CS/VAN@APS-MSN pellet showed no pyrogenic effect and meet the clinical requirements on hemolytic reaction. These results imply that CS/VAN@APS-MSN was an ideal candidate to replace CS bone cement in the treatment of open fractures.
通过将负载万古霉素（VAN）的介孔二氧化硅纳米颗粒（MSN）与硫酸钙半水合物（CS）混合，制备了一种具有万古霉素持续释放功能的新型骨水泥微丸。为了提高VAN的负载能力，用氨丙基三乙氧基硅烷（APS）对MSN进行功能化处理，得到APS-MSN。APS-MSN的VAN负载量和包封率分别可达45.91±0.81%和84.88±1.52%，远高于MSN的负载量（仅为3.91%）和包封率（仅为4.07%）。氮吸附-解吸测量结果表明，大部分VAN存在于APS-MSN的孔隙中。与CS对照微丸相比，CS/VAN@APS-MSN复合微丸显示出强烈的药物缓释效果。体外细胞试验表明，CS/APS-MSN复合材料具有高度的生物相容性，适合用作骨水泥。此外，CS/VAN@APS-MSN微丸无热原效应，符合临床对溶血反应的要求。这些结果表明，CS/VAN@APS-MSN是治疗开放性骨折时替代CS骨水泥的理想候选材料。
