Zhang Peng, Sun Yusheng, Yang Haizhen, Liu Dong, Zhang Feng, Zhang Yu, Zhong Wentao, Zuo Baoqi, Zhou Zhiqiang
Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, China.
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, China.
Front Bioeng Biotechnol. 2023 Jun 1;11:1163933. doi: 10.3389/fbioe.2023.1163933. eCollection 2023.
Chronic osteomyelitis remains a clinical challenge in orthopedics. In this study, silk fibroin microspheres (SFMPs) loaded with vancomycin are entrapped in an injectable silk hydrogel to form a vancomycin delivery system for treatment of chronic osteomyelitis. Vancomycin showed continuous release from the hydrogel for up to 25 days. The hydrogel shows strong antibacterial activity against both Escherichia coli and Staphylococcus aureus and a long antibacterial duration of 10 days without a decrease in the antibacterial effect. The injection of vancomycin-loaded silk fibroin microspheres entrapped in the hydrogel into the infected site of rat tibia reduced bone infection and improved bone regeneration compared with other treatment groups. Thus, the composite SF hydrogel features a sustained-release profile and good biocompatibility, making it promising for application in osteomyelitis treatment.
慢性骨髓炎仍然是骨科领域的一项临床挑战。在本研究中，负载万古霉素的丝素蛋白微球（SFMPs）被包裹在可注射的丝素水凝胶中，形成用于治疗慢性骨髓炎的万古霉素递送系统。万古霉素从水凝胶中持续释放长达25天。该水凝胶对大肠杆菌和金黄色葡萄球菌均表现出强大的抗菌活性，抗菌持续时间长达10天且抗菌效果无下降。与其他治疗组相比，将包裹在水凝胶中的负载万古霉素的丝素蛋白微球注射到大鼠胫骨感染部位可减少骨感染并促进骨再生。因此，复合丝素水凝胶具有缓释特性和良好的生物相容性，使其在骨髓炎治疗中具有应用前景。
