Sorkin Jonathan A, Hughes Stephen, Soares Paulo, Popat Ketul C
Department of Mechanical Engineering, Colorado State University, Fort Collins CO 80523, USA.
Department of Chemical and Biological Engineering, Colorado State University, Fort Collins CO 80523, USA; School of Biomedical Engineering, Colorado State University, Fort Collins CO 80523, USA.
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:735-745. doi: 10.1016/j.msec.2015.01.077. Epub 2015 Jan 26.
Neural prostheses have become ever more acceptable treatments for many different types of neurological damage and disease. Here we investigate the use of two different morphologies of titania nanotube arrays as interfaces to advance the longevity and effectiveness of these prostheses. The nanotube arrays were characterized for their nanotopography, crystallinity, conductivity, wettability, surface mechanical properties and adsorption of key proteins: fibrinogen, albumin and laminin. The loosely packed nanotube arrays fabricated using a diethylene glycol based electrolyte, contained a higher presence of the anatase crystal phase and were subsequently more conductive. These arrays yielded surfaces with higher wettability and lower modulus than the densely packed nanotube arrays fabricated using water based electrolyte. Further the adhesion, proliferation and differentiation of the C17.2 neural stem cell line was investigated on the nanotube arrays. The proliferation ratio of the cells as well as the level of neuronal differentiation was seen to increase on the loosely packed arrays. The results indicate that loosely packed nanotube arrays similar to the ones produced here with a DEG based electrolyte, may provide a favorable template for growth and maintenance of C17.2 neural stem cell line.
神经假体已成为许多不同类型神经损伤和疾病越来越可接受的治疗方法。在此，我们研究了两种不同形态的二氧化钛纳米管阵列作为界面的用途，以提高这些假体的寿命和有效性。对纳米管阵列的纳米形貌、结晶度、导电性、润湿性、表面机械性能以及关键蛋白质（纤维蛋白原、白蛋白和层粘连蛋白）的吸附情况进行了表征。使用基于二甘醇的电解质制备的疏松堆积纳米管阵列含有较高比例的锐钛矿晶相，因此导电性更强。与使用水基电解质制备的紧密堆积纳米管阵列相比，这些阵列产生的表面具有更高的润湿性和更低的模量。此外，还研究了C17.2神经干细胞系在纳米管阵列上的粘附、增殖和分化情况。在疏松堆积的阵列上，细胞的增殖率以及神经元分化水平均有所提高。结果表明，与这里使用基于二甘醇的电解质制备的类似的疏松堆积纳米管阵列，可能为C17.2神经干细胞系的生长和维持提供一个有利的模板。
