柠檬酸杆菌属的多位点序列分析及巴氏柠檬酸杆菌新种的描述
Multilocus sequence analysis of the genus Citrobacter and description of Citrobacter pasteurii sp. nov.
作者信息
Clermont Dominique, Motreff Laurence, Passet Virginie, Fernandez José-Carlos, Bizet Chantal, Brisse Sylvain
机构信息
CIP - Collection de l'Institut Pasteur, Institut Pasteur, Paris, France.
CNRS, UMR 3525, Paris, France.
出版信息
Int J Syst Evol Microbiol. 2015 May;65(Pt 5):1486-1490. doi: 10.1099/ijs.0.000122. Epub 2015 Feb 16.
Strains originating from various sources and classified as members of the genus Citrobacter within the family Enterobacteriaceae were characterized by sequencing internal portions of genes rpoB, fusA, pyrG and leuS, 16S rRNA gene sequencing, average nucleotide identity (ANI) of genomic sequences and biochemical tests. Phylogenetic analysis based on the four housekeeping genes showed that the 11 species of the genus Citrobacter with validly published names are well demarcated. Strains CIP 55.13(T) and CIP 55.9 formed a distinct branch associated with Citrobacter youngae. The ANI between CIP 55.9 and CIP 55.13(T) was 99.19%, whereas it was 94.75% between CIP 55.13(T) and strain CIP 105016(T) of the species C. youngae, the most closely related species. Biochemical characteristics consolidated the fact that the two isolates represent a separate species, for which the name Citrobacter pasteurii sp. nov. is proposed. The type strain is CIP 55.13(T) ( =DSM 28879(T) =Na 1a(T)).
对源自各种来源并归类为肠杆菌科柠檬酸杆菌属成员的菌株，通过对rpoB、fusA、pyrG和leuS基因内部区域进行测序、16S rRNA基因测序、基因组序列的平均核苷酸同一性（ANI）以及生化试验进行特征分析。基于四个管家基因的系统发育分析表明，柠檬酸杆菌属11个有效发表名称的物种界限分明。菌株CIP 55.13(T)和CIP 55.9形成了一个与杨氏柠檬酸杆菌相关的独特分支。CIP 55.9与CIP 55.13(T)之间的ANI为99.19%，而CIP 55.13(T)与最密切相关的杨氏柠檬酸杆菌物种的菌株CIP 105016(T)之间的ANI为94.75%。生化特征证实这两个分离株代表一个单独的物种，为此提出新物种巴氏柠檬酸杆菌的名称。模式菌株为CIP 55.13(T)（=DSM 28879(T)=Na 1a(T)）。
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