Jung Won Joon, Kim Hyoun Joong, Giri Sib Sankar, Kim Sang Guen, Kim Sang Wha, Kang Jeong Woo, Kwon Jun, Lee Sung Bin, Oh Woo Taek, Jun Jin Woo, Park Se Chang
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
Department of Aquaculture, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea.
Microorganisms. 2021 Jan 28;9(2):275. doi: 10.3390/microorganisms9020275.
A novel species was isolated from the kidney of diseased rainbow trout () reared on a trout farm. Biochemical characterization and phylogenetic analysis were performed for bacterial identification. Sequencing of the 16S rRNA gene and five housekeeping genes indicated that the strain belongs to the genus. However, multilocus sequence analysis, a comparison of average nucleotide identity, and genome-to-genome distance values revealed that strain SNU WT2 is distinct and forms a separate clade from other species. Additionally, the phenotype characteristics of the strain differed from those of other species. Quinone analysis indicated that the predominant isoprenoid quinone is Q-10. Furthermore, strain virulence was determined by a rainbow trout challenge trial, and the strain showed resistance to diverse antibiotics including β-lactams, quinolone, and aminoglycosides. The complete genome of strain SNU WT2 is 4,840,504 bp with a DNA G + C content of 51.94% and 106,068-bp plasmid. Genome analysis revealed that the strain carries virulence factors on its chromosome and antibiotic resistance genes on its plasmid. This strain represents a novel species in the genus for which the name has been proposed, with SNU WT2 (=KCTC 72517 = JCM 33612) as the type strain.
从一家鳟鱼养殖场饲养的患病虹鳟鱼（）的肾脏中分离出一个新物种。对该细菌进行了生化特性鉴定和系统发育分析。16S rRNA基因和五个管家基因的测序表明该菌株属于属。然而，多位点序列分析、平均核苷酸同一性比较和全基因组距离值显示，菌株SNU WT2是独特的，与其他物种形成一个单独的进化枝。此外，该菌株的表型特征与其他物种不同。醌分析表明，主要的类异戊二烯醌是Q-10。此外，通过虹鳟鱼攻毒试验确定了菌株的毒力，该菌株对包括β-内酰胺类、喹诺酮类和氨基糖苷类在内的多种抗生素具有抗性。菌株SNU WT2的完整基因组为4,840,504 bp，DNA G + C含量为51.94%，还有一个106,068-bp的质粒。基因组分析表明，该菌株在其染色体上携带毒力因子，在其质粒上携带抗生素抗性基因。该菌株代表属中的一个新物种，已提议将其命名为，以SNU WT2（=KCTC 72517 = JCM 33612）作为模式菌株。
