基于标签化的改良全基因组亚硫酸氢盐测序技术，用于处理少于 100 个哺乳动物细胞的输入 DNA。
Improved tagmentation-based whole-genome bisulfite sequencing for input DNA from less than 100 mammalian cells.
机构信息
BGI-Shenzhen, Shenzhen, China.
出版信息
Epigenomics. 2015;7(1):47-56. doi: 10.2217/epi.14.76.
AIM
To develop a whole-genome methylation sequencing method that fulfills the needs for studies using ultra-low-input DNA.
MATERIALS & METHODS: The tagmentation-based whole-genome bisulfite sequencing (T-WGBS) technology is modified, enabling stable library construction with complexity from minimally 0.5 ng of initial genomic DNA, which equals less than 100 mammalian cells.
RESULTS
We thoroughly assessed the performance of this T-WGBS method by sequencing the methylomes of a rice strain and pre-implantation embryos of rhesus monkey and compare to traditional WGBS approach, thereby demonstrating the efficacy of this new approach.
CONCLUSION
This new approach is highly attractive for the complete methylome analysis of very few cells, for example, mammalian pre-implantation embryos, or tiny human biopsy specimens.
目的
开发一种全基因组甲基化测序方法，以满足使用超低输入量 DNA 进行研究的需求。
材料与方法
对基于标签酶切的全基因组 bisulfite 测序（T-WGBS）技术进行改良，使文库构建具有稳定性，起始基因组 DNA 复杂度低至 0.5ng，相当于少于 100 个哺乳动物细胞。
结果
我们通过对水稻株和恒河猴胚胎植入前的甲基组进行测序，对该 T-WGBS 方法的性能进行了全面评估，并与传统 WGBS 方法进行了比较，从而证明了该新方法的有效性。
结论
对于非常少量的细胞（例如哺乳动物胚胎植入前或人类活检样本）的全甲基化组分析，这种新方法极具吸引力。
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