Christie Andrew E, Chi Megan
Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
Gen Comp Endocrinol. 2015 Mar 1;213:90-109. doi: 10.1016/j.ygcen.2015.02.003. Epub 2015 Feb 14.
The public deposition of large transcriptome shotgun assembly (TSA) datasets for the Araneae (true spiders) provides a resource for determining the structures of the native neuropeptides present in members of this chelicerate order. Here, the Araneae TSA data were mined for putative peptide-encoding transcripts using the recently deduced neuropeptide precursors from the Western black widow Latrodectus hesperus as query templates. Neuropeptide-encoding transcripts from five spiders, Latrodectus tredecimguttatus, Stegodyphus mimosarum, Stegodyphus lineatus, Stegodyphus tentoriicola and Acanthoscurria geniculata, were identified, including ones encoding members of the allatostatin A, allatostatin B, allatostatin C, allatotropin, CAPA/periviscerokinin/pyrokinin, crustacean cardioactive peptide, crustacean hyperglycemic hormone/ion transport peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, FMRFamide-like peptide (FLP), GSEFLamide, insulin-like peptide, orcokinin, proctolin, short neuropeptide F, SIFamide, sulfakinin and tachykinin-related peptide (TRP) families. A total of 156 distinct peptides were predicted from the precursor proteins deduced from the S. mimosarum transcripts, with 65, 26, 21 and 12 peptides predicted from those deduced from the A. geniculata, L. tredecimguttatus, S. lineatus and S. tentoriicola sequences, respectively. Among the peptides identified were variant isoforms of FLP, orcokinin and TRP, peptides whose structures are similar to ones previously identified from L. hesperus. The prediction of these atypical peptides from multiple spiders suggests that they may be broadly conserved within the Araneae rather than being species-specific variants. Taken collectively, the data described here greatly expand the number of known Araneae neuropeptides, providing a foundation for future functional studies of peptidergic signaling in this important Chelicerate order.
蜘蛛目（真蜘蛛）大型转录组鸟枪法装配（TSA）数据集的公开存档为确定该螯肢动物目成员中存在的天然神经肽结构提供了资源。在此，以最近从西部黑寡妇间斑寇蛛推导的神经肽前体作为查询模板，在蜘蛛目TSA数据中挖掘推定的肽编码转录本。鉴定出了来自五种蜘蛛的神经肽编码转录本，这五种蜘蛛分别是红斑寇蛛、刺足蛛、条纹刺足蛛、帐篷刺足蛛和膝形棘蛛，包括编码咽侧体抑制素A、咽侧体抑制素B、咽侧体抑制素C、促咽侧体素、CAPA/围脏神经激肽/速激肽、甲壳类心脏活性肽、甲壳类高血糖激素/离子转运肽、利尿激素31、利尿激素44、蜕皮激素、FMRF酰胺样肽（FLP）、GSEFL酰胺、胰岛素样肽、促心肽、促肠肽、短神经肽F、SIF酰胺、磺基激肽和速激肽相关肽（TRP）家族成员的转录本。从刺足蛛转录本推导的前体蛋白中总共预测出156种不同的肽，从膝形棘蛛、红斑寇蛛、条纹刺足蛛和帐篷刺足蛛序列推导的前体蛋白中分别预测出65、26、21和12种肽。鉴定出的肽中包括FLP、促心肽和TRP的变体亚型，其结构与先前从间斑寇蛛中鉴定出的相似。从多种蜘蛛中预测出这些非典型肽表明它们可能在蜘蛛目中广泛保守，而不是物种特异性变体。总体而言，此处描述的数据极大地扩展了已知的蜘蛛目神经肽数量，为该重要螯肢动物目肽能信号传导未来功能研究奠定了基础。
