Chai Wei, Zhang Zhendong, Ni Ming, Geng Peiliang, Lian Zijian, Zhang Guoqiang, Shi Lewis L, Chen Jiying
Department of Orthopaedics, Chinese People's Liberation Army General Hospital, 301 Orthopaedic Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.
Cancer Center, Division of Internal Medicine, Chinese PLA General Hospital & Chinese PLA Medical School, 28 Fuxing Road, Haidian District, Beijing 100853, China.
Biomed Res Int. 2015;2015:196495. doi: 10.1155/2015/196495. Epub 2015 Jan 26.
Methylenetetrahydrofolate reductase (MTHFR) SNP rs1801133 has been frequently investigated in recent years. Relevant candidate gene association studies with this SNP and osteonecrosis of the femoral head (ONFH) reported conflicting results. Meta-analysis provides a method to combine these data and to determine the association in a larger sample size.
We conducted a systematic search to identify possible studies. Four pooled ORs (odds ratios, T versus C, TT versus CC, TT/CT versus CC, and TT versus CT/CC), along with 95% confidence interval (CI), were calculated to evaluate the association between SNP rs1801133 and ONFH susceptibility. Both fixed effects model and random effects model were used.
We eventually included twelve studies in this analysis, with results showing no overall association between ONFH susceptibility and SNP rs1801133 (T versus C: OR=1.15, 95% CI=0.97-1.38; TT versus CC: OR=1.15, 95% CI=0.91-1.46; TT/CT versus CC: OR=1.09, 95% CI=0.95-1.25; and TT versus
CT/CC: OR=1.16, 95% CI=0.93-1.45). When stratified based on ethnicity, the results were still not significant.
Our findings are generally supportive of no association between MTHFR SNP rs1801133 and the etiology of ONFH.
近年来，亚甲基四氢叶酸还原酶（MTHFR）单核苷酸多态性（SNP）rs1801133受到了频繁研究。关于该SNP与股骨头坏死（ONFH）的相关候选基因关联研究报告了相互矛盾的结果。荟萃分析提供了一种合并这些数据并在更大样本量中确定关联的方法。
我们进行了系统检索以识别可能的研究。计算了四个合并的比值比（优势比，T对C、TT对CC、TT/CT对CC以及TT对CT/CC）以及95%置信区间（CI），以评估SNP rs1801133与ONFH易感性之间的关联。同时使用了固定效应模型和随机效应模型。
我们最终纳入了12项研究进行此分析，结果显示ONFH易感性与SNP rs1801133之间无总体关联（T对C：OR = 1.15，95% CI = 0.97 - 1.38；TT对CC：OR = 1.15，95% CI = 0.91 - 1.46；TT/CT对CC：OR = 1.09，95% CI = 0.95 - 1.25；TT对CT/CC：OR = 1.16，95% CI = 0.93 - 1.45）。按种族分层时，结果仍然不显著。
我们的研究结果总体上支持MTHFR SNP rs1801133与ONFH病因之间无关联。
