Lange P, Greco K, Partington L, Carvalho C, Oliani S, Birchall M A, Sibbons P D, Lowdell M W, Ansari T
Department of Surgical Research, NPIMR, Watford Rd, Harrow, UK.
Department of Haematology, UCL, Medical School, London, UK.
J Tissue Eng Regen Med. 2017 Mar;11(3):800-811. doi: 10.1002/term.1979. Epub 2015 Feb 17.
Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum technology, which when combined with an enzyme/detergent protocol significantly reduces the time required to create clinically suitable scaffolds. We have applied this technology to prepare porcine tracheal scaffolds and compared the results to scaffolds produced under normal atmospheric pressures. The principal outcome measures were the reduction in time (9 days to prepare the scaffold) followed by a reduction in residual DNA levels (DC no-vac: 137.8±48.82 ng/mg vs. DC vac 36.83±18.45 ng/mg, p<0.05.). Our approach did not impact on the collagen or glycosaminoglycan content or on the biomechanical properties of the scaffolds. We applied the vacuum technology to human tracheae, which, when implanted in vivo showed no significant adverse immunological response. The addition of a vacuum to a conventional decellularization protocol significantly reduces production time, whilst providing a suitable scaffold. This increases clinical utility and lowers production costs. To our knowledge this is the first time that vacuum assisted decellularization has been explored. Copyright © 2015 John Wiley & Sons, Ltd.
基于人道主义理由，组织工程气管已成功植入少数患者体内进行治疗。由于制备支架所需的时间以及由此产生的财务成本，这种治疗方法尚未成为主流。我们开发了一种基于真空技术的去细胞化（DC）方法，当与酶/去污剂方案结合使用时，可显著减少制备临床适用支架所需的时间。我们已将该技术应用于制备猪气管支架，并将结果与在正常大气压下制备的支架进行了比较。主要结果指标是时间的减少（制备支架的时间从9天减少），其次是残留DNA水平的降低（DC无真空：137.8±48.82 ng/mg，而DC真空：36.83±18.45 ng/mg，p<0.05）。我们的方法对支架的胶原蛋白或糖胺聚糖含量以及生物力学性能没有影响。我们将真空技术应用于人体气管，当将其植入体内时未显示出明显的不良免疫反应。在传统的去细胞化方案中加入真空可显著减少生产时间，同时提供合适的支架。这增加了临床实用性并降低了生产成本。据我们所知，这是首次探索真空辅助去细胞化。版权所有©2015约翰威立父子有限公司。
