Shih Jheng-Hong, Chern Edward
niChe Lab for Stem Cell and Regenerative Medicine, Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, 10617, Taiwan.
Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, No. 1, Sec. 4 Roosevelt Road, Taipei, 10617, Taiwan.
Stem Cell Rev Rep. 2025 Apr 14. doi: 10.1007/s12015-025-10875-y.
Tissue engineering has been an integral part of regenerative medicine. Functional biomimetic structures were assembled by combining appropriate scaffolds with specific cells. The decellularization of animal tissue preserved the natural biochemical components and structural properties of the extracellular matrix (ECM) of specific organs, thereby providing a suitable niche for tissue-specific cell differentiation and growth. In this study, the extracellular matrix (ECM) of the porcine aorta was obtained through trypsin-based decellularization. The resulting porcine aortic ECM retained a favorable collagen composition, exhibited no cytotoxicity, and demonstrated chemophilic properties for mesenchymal stem cells. Human adipose-derived mesenchymal stem cells (hADSCs) and human induced pluripotent stem cell-derived mesenchymal stem cells (hiMSCs) were transplanted onto the decellularized porcine aortic ECM, where successful differentiation into a mature cartilage layer was observed. These findings suggested that the porcine aortic ECM could serve as a potential scaffold with tubular and linear structures for tissue engineering applications. Functional human iMSCs (induced-mesenchymal stem cells) were generated from human iPSCs (induced-pluripotent stem cells) and analyzed for differences compared to primary MSCs via RNA-seq. The hiMSCs were applied to decellularized porcine aortic ECM (extracellular matrix), demonstrating chondrogenic differentiation and confirming the usability of xenogeneic ECM.
组织工程一直是再生医学的一个组成部分。通过将合适的支架与特定细胞相结合来组装功能性仿生结构。动物组织的去细胞化保留了特定器官细胞外基质（ECM）的天然生化成分和结构特性，从而为组织特异性细胞分化和生长提供了合适的微环境。在本研究中，通过基于胰蛋白酶的去细胞化方法获得了猪主动脉的细胞外基质（ECM）。所得的猪主动脉ECM保留了良好的胶原蛋白组成，无细胞毒性，并对间充质干细胞表现出趋化特性。将人脂肪来源的间充质干细胞（hADSCs）和人诱导多能干细胞来源的间充质干细胞（hiMSCs）移植到去细胞化的猪主动脉ECM上，观察到它们成功分化为成熟的软骨层。这些发现表明，猪主动脉ECM可作为一种具有管状和线性结构的潜在支架用于组织工程应用。功能性人诱导间充质干细胞（iMSCs）由人诱导多能干细胞（iPSCs）产生，并通过RNA测序分析与原代间充质干细胞的差异。将hiMSCs应用于去细胞化的猪主动脉细胞外基质（ECM），证明其软骨分化能力并证实了异种ECM的可用性。
