Viet-Nhi Nguyen-Kieu, Chen Yen-Chun, Dang Luong Huu, Tseng How, Hung Shih-Han
International Master/Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Department of Otolaryngology, Taipei Medical University Hospital, Taipei 110, Taiwan.
J Funct Biomater. 2023 Mar 5;14(3):147. doi: 10.3390/jfb14030147.
Few efforts have been made regarding the optimization of porcine small intestinal submucosa (SIS) to improve its biocompatibility. This study aims to evaluate the effect of SIS degassing on the promotion of cell attachment and wound healing. The degassed SIS was evaluated in vitro and in vivo, compared with the nondegassed SIS control. In the cell sheet reattachment model, the reattached cell sheet coverage was significantly higher in the degassed SIS group than in the nondegassed group. Cell sheet viability was also significantly higher in the SIS group than in the control group. In vivo studies showed that the tracheal defect repaired by the degassed SIS patch showed enhanced healing and reductions in fibrosis and luminal stenosis compared to the nondegassed SIS control group, with the thickness of the transplanted grafts in the degassed SIS group significantly lower than those in the control group (346.82 ± 28.02 µm vs. 771.29 ± 20.41 µm, < 0.05). Degassing the SIS mesh significantly promoted cell sheet attachment and wound healing by reducing luminal fibrosis and stenosis compared to the nondegassed control SIS. The results suggest that the degassing processing might be a simple and effective way to improve the biocompatibility of SIS.
在优化猪小肠黏膜下层（SIS）以提高其生物相容性方面所做的努力很少。本研究旨在评估SIS脱气对促进细胞附着和伤口愈合的影响。将脱气后的SIS与未脱气的SIS对照进行体外和体内评估。在细胞片重新附着模型中，脱气SIS组的重新附着细胞片覆盖率显著高于未脱气组。SIS组的细胞片活力也显著高于对照组。体内研究表明，与未脱气的SIS对照组相比，用脱气SIS补片修复的气管缺损显示出愈合增强，纤维化和管腔狭窄减少，脱气SIS组移植移植物的厚度显著低于对照组（346.82±28.02µm对771.29±20.41µm，<0.05）。与未脱气的对照SIS相比，对SIS网进行脱气可通过减少管腔纤维化和狭窄显著促进细胞片附着和伤口愈合。结果表明，脱气处理可能是一种改善SIS生物相容性的简单有效方法。
