Huang Minjie, Zhu Huijun, Feng Jian, Ni Songshi, Huang Jianfei
Department of Respiratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
Dis Markers. 2015;2015:986095. doi: 10.1155/2015/986095. Epub 2015 Jan 18.
The aim of this study was to investigate the expression of Prominin-1 (CD133) in cancer cells and its potential value as a prognostic indicator of survival in patients with non-small cell lung cancer (NSCLC).
Cancerous tissues and matched normal tissues adjacent to the carcinoma from 239 NSCLC patients were obtained immediately after surgery. Immunohistochemistry of tissue microarrays was used to characterize the expression of CD133 in NSCLC and adjacent tissues. The correlation of CD133 expression with clinical characteristics and prognosis was determined by statistical analysis.
CD133 protein expression levels in both the cytoplasm and nucleus were significantly higher in NSCLC tissues compared with corresponding peritumoral tissue (P < 0.05). CD133 expression in the nucleus of NSCLC cells was related to tumor diameter (P = 0.027), tumor differentiation (P < 0.001), and TNM stage (P = 0.007). Kaplan-Meier survival and Cox regression analyses revealed that high CD133 expression in the nucleus was an independent predictor of poor prognosis of NSCLC, as was high cytoplasmic CD133 expression (P < 0.001).
Our findings provide the first evidence that high expression of CD133 in both the nucleus and cytoplasm is associated with poor prognosis in NSCLC.
本研究旨在调查Prominin-1（CD133）在癌细胞中的表达及其作为非小细胞肺癌（NSCLC）患者生存预后指标的潜在价值。
239例NSCLC患者术后立即获取癌组织及与之匹配的癌旁正常组织。采用组织芯片免疫组化法检测NSCLC组织及癌旁组织中CD133的表达情况。通过统计学分析确定CD133表达与临床特征及预后的相关性。
与相应癌旁组织相比，NSCLC组织中细胞质和细胞核内的CD133蛋白表达水平均显著升高（P < 0.05）。NSCLC细胞核内CD133表达与肿瘤直径（P = 0.027）、肿瘤分化程度（P < 0.001）及TNM分期（P = 0.007）相关。Kaplan-Meier生存分析和Cox回归分析显示，细胞核内高表达CD133是NSCLC预后不良的独立预测指标，细胞质内高表达CD133亦是如此（P < 0.001）。
我们的研究结果首次证明，细胞核和细胞质内CD133高表达均与NSCLC预后不良相关。
