Wallace Bret D, Williams R Scott
a Genome Integrity and Structural Biology Laboratory; National Institute of Environmental Health Sciences; NIH; DHHS ; Research Triangle Park , NC USA.
RNA Biol. 2014;11(11):1340-6. doi: 10.4161/15476286.2014.992283.
Research indicates that the transient contamination of DNA with ribonucleotides exceeds all other known types of DNA damage combined. The consequences of ribose incorporation into DNA, and the identity of protein factors operating in this RNA-DNA realm to protect genomic integrity from RNA-triggered events are emerging. Left unrepaired, the presence of ribonucleotides in genomic DNA impacts cellular proliferation and is associated with chromosome instability, gross chromosomal rearrangements, mutagenesis, and production of previously unrecognized forms of ribonucleotide-triggered DNA damage. Here, we highlight recent findings on the nature and structure of DNA damage arising from ribonucleotides in DNA, and the identification of cellular factors acting in an RNA-DNA damage response (RDDR) to counter RNA-triggered DNA damage.
研究表明，DNA被核糖核苷酸短暂污染的情况超过了所有其他已知类型的DNA损伤之和。核糖掺入DNA的后果，以及在这个RNA-DNA领域中发挥作用以保护基因组完整性免受RNA引发事件影响的蛋白质因子的身份正在逐渐明晰。如果不进行修复，基因组DNA中核糖核苷酸的存在会影响细胞增殖，并与染色体不稳定、染色体大片段重排、诱变以及产生以前未被认识的核糖核苷酸引发的DNA损伤形式有关。在这里，我们重点介绍了关于DNA中核糖核苷酸引起的DNA损伤的性质和结构的最新发现，以及鉴定在RNA-DNA损伤反应（RDDR）中起作用以对抗RNA引发的DNA损伤的细胞因子。
