Mosquera Jesús, Sánchez Mateo I, Valero Julián, de Mendoza Javier, Vázquez M Eugenio, Mascareñas José L
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), C/ Jenaro de la Fuente s/n, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Chem Commun (Camb). 2015 Mar 21;51(23):4811-4. doi: 10.1039/c4cc09525a.
Conjugation of a short peptide fragment from a bZIP protein to an oligoguanidinium tail results in a DNA-binding miniprotein that selectively interacts with composite sequences containing the peptide-binding site next to an A/T-rich tract. In addition to stabilizing the complex with the target DNA, the oligoguanidinium unit also endows the conjugate with cell internalization properties.
将来自碱性亮氨酸拉链（bZIP）蛋白的短肽片段与低聚胍尾缀合，会产生一种DNA结合小蛋白，该蛋白能与富含A/T序列旁包含肽结合位点的复合序列选择性相互作用。除了稳定与靶DNA的复合物外，低聚胍单元还赋予缀合物细胞内化特性。
