Sacco Pasquale, Travan Andrea, Borgogna Massimiliano, Paoletti Sergio, Marsich Eleonora
Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127, Trieste, Italy,
J Mater Sci Mater Med. 2015 Mar;26(3):128. doi: 10.1007/s10856-015-5474-7. Epub 2015 Feb 19.
Treatment of non-healing wounds represents hitherto a severe dilemma because of their failure to heal caused by repeated tissue insults, bacteria contamination and altered physiological condition. This leads to face huge costs for the healthcare worldwide. To this end, the development of innovative biomaterials capable of preventing bacterial infection, of draining exudates and of favoring wound healing is very challenging. In this study, we exploit a novel technique based on the slow diffusion of tripolyphosphate for the preparation of macroscopic chitosan hydrogels to obtain soft pliable membranes which include antimicrobial silver nanoparticles (AgNPs) stabilized by a lactose-modified chitosan (Chitlac). UV-Vis and TEM analyses demonstrated the time stability and the uniform distribution of AgNPs in the gelling mixture, while swelling studies indicated the hydrophilic behavior of membrane. A thorough investigation on bactericidal properties of the material pointed out the synergistic activity of chitosan and AgNPs to reduce the growth of S. aureus, E. coli, S. epidermidis, P. aeruginosa strains and to break apart mature biofilms. Finally, biocompatibility assays on keratinocytes and fibroblasts did not prove any harmful effects on the viability of cells. This novel technique enables the production of bioactive membranes with great potential for the treatment of non-healing wounds.
由于反复的组织损伤、细菌污染和生理状况改变导致伤口无法愈合，迄今为止，治疗难愈合伤口是一个严峻的难题。这导致全球医疗保健面临巨大成本。为此，开发能够预防细菌感染、引流渗出液并促进伤口愈合的创新生物材料极具挑战性。在本研究中，我们利用一种基于三聚磷酸缓慢扩散的新技术制备宏观壳聚糖水凝胶，以获得柔软易弯曲的膜，其中包含由乳糖修饰的壳聚糖（Chitlac）稳定的抗菌银纳米颗粒（AgNPs）。紫外可见光谱和透射电子显微镜分析证明了AgNPs在凝胶混合物中的时间稳定性和均匀分布，而溶胀研究表明了膜的亲水性。对该材料杀菌性能的深入研究指出，壳聚糖和AgNPs具有协同活性，可减少金黄色葡萄球菌、大肠杆菌、表皮葡萄球菌、铜绿假单胞菌菌株的生长，并分解成熟的生物膜。最后，对角质形成细胞和成纤维细胞的生物相容性测定未证明对细胞活力有任何有害影响。这种新技术能够生产具有巨大潜力的生物活性膜，用于治疗难愈合伤口。
