Ohtani Misato
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara, 630-0192, Japan,
J Plant Res. 2015 May;128(3):361-9. doi: 10.1007/s10265-015-0700-4. Epub 2015 Feb 19.
The characteristic high regenerative ability of plants has been exploited to develop in vitro plant regeneration techniques, which are usually initiated by an in vitro dedifferentiation step induced by artificial phytohormone treatment. Recent advances in plant molecular biological and genetic technologies have revealed the importance of the regulation of RNA metabolism, including the control of rRNA biosynthesis, pre-mRNA splicing, and miRNA-based RNA decay, in successful in vitro dedifferentiation. This review provides a brief overview of current knowledge of the roles of RNA metabolism in the dedifferentiation of plant cells in vitro. In addition, the possibility that plant-specific aspects of RNA metabolism regulation are linked closely to their high regenerative ability is discussed.
植物具有的高再生能力已被用于开发体外植物再生技术，这些技术通常由人工植物激素处理诱导的体外去分化步骤启动。植物分子生物学和遗传技术的最新进展揭示了RNA代谢调控在成功的体外去分化中的重要性，包括对rRNA生物合成、前体mRNA剪接和基于miRNA的RNA降解的控制。本文综述简要概述了目前关于RNA代谢在植物细胞体外去分化中作用的知识。此外，还讨论了RNA代谢调控的植物特异性方面与其高再生能力密切相关的可能性。
