Juffermans L J M, Dijkmans P A, Musters R J P, van Wamel A, Bouakaz A, Ten Cate F J, Deelman L, Visser C A, de Jong N, Kamp O
Neth Heart J. 2004 Sep;12(9):394-399.
Although gene therapy has great potential as a treatment for diseases, clinical trials are slowed down by the development of a safe and efficient gene delivery system. In this review, we will give an overview of the viral and nonviral vehicles used for drug and gene delivery, and the different nonviral delivery techniques, thereby focusing on delivery through ultrasound contrast agents. The development of ultrasound contrast agents containing encapsulated microbubbles has increased the possibilities not only for diagnostic imaging, but for therapy as well. Microbubbles have been shown to be able to carry drugs and genes, and destruction of the bubbles by ultrasound will result in local release of their contents. Furthermore, ligands can be attached so that they can be targeted to a specific target tissue. The recent advances of microbubbles as vehicles for delivery of drugs and genes will be highlighted.
尽管基因治疗作为一种疾病治疗方法具有巨大潜力，但安全高效的基因递送系统的发展减缓了临床试验的进程。在本综述中，我们将概述用于药物和基因递送的病毒和非病毒载体，以及不同的非病毒递送技术，从而聚焦于通过超声造影剂进行的递送。含有包封微泡的超声造影剂的发展不仅增加了诊断成像的可能性，也增加了治疗的可能性。微泡已被证明能够携带药物和基因，超声对微泡的破坏将导致其内容物的局部释放。此外，可以连接配体，使其能够靶向特定的靶组织。将重点介绍微泡作为药物和基因递送载体的最新进展。
