Hahn S, Buratowski S, Sharp P A, Guarente L
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5718-22. doi: 10.1073/pnas.86.15.5718.
The DNA binding properties of the yeast TATA element-binding protein TFIID were investigated. The affinity (apparent equilibrium dissociation constant) of TFIID for the adenovirus major late promoter consensus TATA element is 2 x 10(-9) M, a value similar to the affinity of gene-specific regulatory proteins for their binding sites. TFIID binding is highly specific and recognizes nonspecific sites with approximately 10(5)-fold lower affinity. Despite this specificity, TFIID also binds with high affinity to several TATA elements that do not match the consensus TATA sequences (TATAAA and TATATA): the yeast LEU2 TATA (TATTATTTA), the simian virus 40 TATA (CTTATTTAT), and the yeast CYC1 -10 TATA (TTATACATT) all bound TFIID. Furthermore, TFIID was active in promoting transcription in vitro from the nonconsensus TATA elements. Thus, contrary to previous suggestions, the existence of nonconsensus TATA elements does not itself indicate the existence of multiple TATA-binding factors.
对酵母TATA元件结合蛋白TFIID的DNA结合特性进行了研究。TFIID对腺病毒主要晚期启动子共有TATA元件的亲和力（表观平衡解离常数）为2×10⁻⁹ M，该值与基因特异性调节蛋白对其结合位点的亲和力相似。TFIID的结合具有高度特异性，对非特异性位点的识别亲和力约低10⁵倍。尽管具有这种特异性，但TFIID也以高亲和力与几个与共有TATA序列（TATAAA和TATATA）不匹配的TATA元件结合：酵母LEU2 TATA（TATTATTTA）、猿猴病毒40 TATA（CTTATTTAT）和酵母CYC1 -10 TATA（TTATACATT）均能结合TFIID。此外，TFIID在体外能促进非共有TATA元件的转录。因此，与之前的观点相反，非共有TATA元件的存在本身并不表明存在多种TATA结合因子。
