Horikoshi M, Bertuccioli C, Takada R, Wang J, Yamamoto T, Roeder R G
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1060-4. doi: 10.1073/pnas.89.3.1060.
The TATA box-binding factor TFIID plays a primary role in the process of transcription initiation by RNA polymerase II and its regulation by various gene-specific factors. Here we employ a permuted binding site/gel retardation assay with recombinant yeast and human TFIID to show that this factor induces DNA bending around the TATA element. These results are consistent with the presence of G + C-rich sequence elements flanking the consensus TATA element and led to the recently confirmed suggestion that TFIID interacts with the TATA element via the minor groove. They also raise the possibility that TFIID-induced bending might facilitate promoter interactions of other general factors in the preinitiation complex or interactions between general transcription factors and regulatory factors bound at upstream sites.
TATA 框结合因子 TFIID 在 RNA 聚合酶 II 转录起始过程及其受各种基因特异性因子调控的过程中发挥着主要作用。在此，我们利用重组酵母和人 TFIID 进行置换结合位点/凝胶阻滞分析，以表明该因子可诱导围绕 TATA 元件的 DNA 弯曲。这些结果与共有 TATA 元件侧翼富含 G + C 的序列元件的存在相一致，并导致最近得到证实的推测，即 TFIID 通过小沟与 TATA 元件相互作用。它们还提出了一种可能性，即 TFIID 诱导的弯曲可能促进起始前复合物中其他通用因子的启动子相互作用，或通用转录因子与结合在上游位点的调控因子之间的相互作用。
