Vázquez-Frias R, Gutiérrez-Reyes G, Urbán-Reyes M, Velázquez-Guadarrama N, Fortoul-van der Goes T I, Reyes-López A, Consuelo-Sánchez A
Departamento de Gastroenterología y Nutrición, Hospital Infantil de México Federico Gómez, SSA, México D.F., México.
Laboratorio de Hígado Páncreas y Motilidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., México.
Rev Gastroenterol Mex. 2015 Jan-Mar;80(1):6-12. doi: 10.1016/j.rgmx.2014.11.001. Epub 2015 Feb 16.
There is evidence that patients with irritable bowel syndrome (IBS) have a low degree of inflammation in the intestinal mucosa. The aim of the study was to evaluate the profile of pro- and anti-inflammatory cytokines in plasma in Mexican pediatric patients with IBS.
Fifteen patients with IBS according to Rome III criteria for childhood and 15 healthy children, matched by age and sex, were included in the study. Plasma levels of tumoral necrosis factor alpha (TNF-α), interleukins 10 and 12 (IL-10, IL-12) and transforming growth factor beta (TGF-β) were quantified and compared between groups.
Plasma levels of IL-10 were lower in patients with IBS (86.07+21.3 pg/mL vs. 118.71+58.62 pg/mL: P=.045) and IL-12 levels were higher in patients with IBS compared to the control group of healthy children (1,204.2±585.9 pg/mL vs. 655.04±557.80 pg/mL; P=.011). The IL-10/IL-12 index was lower in patients with IBS (0.097±0.07 vs. 0.295±0.336; P=.025). Plasma concentration of TGF-β was higher in patients with IBS (545.67±337.69 pg/mL vs. 208.48±142.21 pg/mL; P=.001). There was no difference in plasma levels of TNF-α between groups.
This study suggests that children with IBS have a state of altered immune regulation. This is consistent with the theory of low-grade inflammatory state in these patients. Further studies are needed to elucidate the role played by these cytokines, specifically TGF-β in the pathogenesis of IBS.
有证据表明，肠易激综合征（IBS）患者的肠黏膜存在低度炎症。本研究旨在评估墨西哥患IBS的儿科患者血浆中促炎和抗炎细胞因子的情况。
本研究纳入了15名符合儿童罗马III标准的IBS患者以及15名年龄和性别相匹配的健康儿童。对两组患者的血浆肿瘤坏死因子α（TNF-α）、白细胞介素10和12（IL-10、IL-12）以及转化生长因子β（TGF-β）水平进行定量并比较。
IBS患者的血浆IL-10水平较低（86.07 + 21.3 pg/mL 对比 118.71 + 58.62 pg/mL：P = 0.045），且IBS患者的IL-12水平高于健康儿童对照组（1204.2 ± 585.9 pg/mL 对比 655.04 ± 557.80 pg/mL；P = 0.011）。IBS患者的IL-10/IL-12指数较低（0.097 ± 0.07 对比 0.295 ± 0.336；P = 0.025）。IBS患者的血浆TGF-β浓度较高（545.67 ± 337.69 pg/mL 对比 208.48 ± 142.21 pg/mL；P = 0.001）。两组之间的血浆TNF-α水平无差异。
本研究表明，患IBS的儿童存在免疫调节改变的状态。这与这些患者的低度炎症状态理论相符。需要进一步研究以阐明这些细胞因子，特别是TGF-β在IBS发病机制中所起的作用。
