Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology sciences, Kerman University of medical Sciences, Kerman, Iran; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Clin Res Hepatol Gastroenterol. 2016 Nov;40(5):631-637. doi: 10.1016/j.clinre.2015.12.013. Epub 2016 Feb 2.
Irritable bowel syndrome (IBS) is the most prevalent functional gastrointestinal (GI) disorder, which presents with abdominal pain and changes in the bowel habits. Although the exact cause of IBS remains uncertain, some studies have shown that the inflammation and cytokine imbalance may act as potential etiological factors. The aim of our study is to compare the serum levels of interleukin 6 (IL-6), interleukin 8 (IL-8), and tumor necrosis factor-alpha (TNF-α) in patients with IBS with the healthy controls. The other aim of this study is to evaluate possible association between above-mentioned cytokines and IBS subtypes.
Seventy-four IBS patients diagnosed based on Rome III criteria and 75 gender and age-matched healthy controls were included in this study. Cytokines were measured in the serum using enzyme-linked immunosorbent assays (ELISA).
Patients were classified into groups of IBS with diarrhea (IBS-D): 34, IBS with constipation (IBS-C): 29, and IBS with mixed symptoms (IBS-M): 11. The serum levels of IL-6, IL-8 and TNF-α were significantly higher in patients with IBS as compared to controls (P<0.001). There was no difference in serum levels of cytokines based on IBS subtypes.
Higher serum level of IL-6, IL-8 and TNF-α in IBS suggests an important role of cytokines as immune mediators in the pathogenesis of this functional GI disorder. To understand any association between cytokines and IBS subtypes, further investigations with larger sample sizes are desired.
肠易激综合征（IBS）是最常见的功能性胃肠道（GI）疾病，其特征为腹痛和肠道习惯改变。尽管 IBS 的确切病因仍不清楚，但一些研究表明炎症和细胞因子失衡可能是潜在的病因。我们的研究目的是比较 IBS 患者和健康对照者的血清白细胞介素 6（IL-6）、白细胞介素 8（IL-8）和肿瘤坏死因子-α（TNF-α）水平。本研究的另一个目的是评估上述细胞因子与 IBS 亚型之间的可能关联。
本研究纳入了 74 例根据 Rome III 标准诊断的 IBS 患者和 75 名性别和年龄匹配的健康对照者。使用酶联免疫吸附试验（ELISA）测量血清中的细胞因子。
患者被分为 IBS 腹泻型（IBS-D）组：34 例、IBS 便秘型（IBS-C）组：29 例和 IBS 混合症状型（IBS-M）组：11 例。与对照组相比，IBS 患者的血清 IL-6、IL-8 和 TNF-α 水平显著升高（P<0.001）。根据 IBS 亚型，细胞因子的血清水平没有差异。
IBS 患者血清中 IL-6、IL-8 和 TNF-α 水平升高提示细胞因子作为免疫介质在这种功能性 GI 疾病的发病机制中起重要作用。为了了解细胞因子与 IBS 亚型之间的任何关联，需要进行更大样本量的进一步研究。
