Wang C T
Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Jpn J Vet Res. 1991 Dec;39(2-4):107-15.
I evaluated an enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) test simultaneously for the detection of bovine leukemia virus (BLV) antibodies. Total 1,293 serum samples were tested for ELISA and AGID test and the results were compared. The results of ELISA and AGID agreed by 1,156 out of 1,293 (89.4%). All of AGID-positive 356 sera were positive by ELISA. However, of 451 ELISA-positive sera, 95 sera were either negative or equivocal by AGID test. Eleven animals which showed ELISA-positive but AGID-negative or equivocal became AGID-positive in a year. It may be inferred that ELISA detects infected cattle earlier and with greater sensitivity than AGID.
我同时评估了酶联免疫吸附测定（ELISA）和琼脂凝胶免疫扩散（AGID）试验用于检测牛白血病病毒（BLV）抗体的情况。总共1293份血清样本进行了ELISA和AGID试验，并对结果进行了比较。ELISA和AGID的结果在1293份样本中有1156份一致（89.4%）。所有356份AGID阳性血清通过ELISA检测均为阳性。然而，在451份ELISA阳性血清中，有95份血清通过AGID试验检测为阴性或可疑。11只ELISA检测为阳性但AGID检测为阴性或可疑的动物在一年内AGID检测变为阳性。可以推断，ELISA比AGID能更早且更灵敏地检测出感染牛。
