Arribas Jéssica, Castellví Josep, Marcos Ricard, Zafón Carles, Velázquez Antonia
Grup de Mutagènesi, Unitat de Genètica, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallés, Barcelona, Spain.
Endocr Pathol. 2015 May;26(2):111-8. doi: 10.1007/s12022-015-9359-6.
The transcription factor Yin Yang 1 (YY1) has an important regulatory role in tumorigenesis, but its implication in thyroid cancer has not been yet investigated. In the present study, we have analyzed the expression of YY1 in differentiated thyroid cancer and assessed the association of YY1 expression with clinical features. Expression of YY1 was evaluated in human thyroid cancer cell lines, a series of matched normal/tumor thyroid tissues and in a thyroid cancer tissue microarray, using real-time PCR, Western blot, and/or immunohistochemistry. YY1 was overexpressed in thyroid cancer cells, at transcription and protein levels. A significant increase of YY1 mRNA was also observed in tumor thyroid tissues. Moreover, immunohistochemical analysis of the thyroid cancer tissue microarray revealed that both papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC) present increased YY1 protein levels (48 and 19%, respectively). After stratification by the level of YY1 protein, positive YY1 expression identifies 88% of patients with PTC. The association of YY1 expression with clinicopathological features in PTC and FTC showed that YY1 expression was related with age at diagnosis. Our data indicates for the first time overexpression of YY1 in differentiated thyroid cancer, with YY1 being more frequently overexpressed in the PTC subtype.
转录因子阴阳1（YY1）在肿瘤发生过程中发挥着重要的调控作用，但其在甲状腺癌中的作用尚未得到研究。在本研究中，我们分析了YY1在分化型甲状腺癌中的表达情况，并评估了YY1表达与临床特征之间的关联。我们采用实时定量聚合酶链反应、蛋白质免疫印迹法和/或免疫组织化学法，对人甲状腺癌细胞系、一系列配对的正常/肿瘤甲状腺组织以及一个甲状腺癌组织芯片中YY1的表达进行了评估。YY1在甲状腺癌细胞的转录水平和蛋白质水平均呈过表达状态。在肿瘤甲状腺组织中也观察到YY1信使核糖核酸显著增加。此外，对甲状腺癌组织芯片进行免疫组织化学分析显示，甲状腺乳头状癌（PTC）和甲状腺滤泡状癌（FTC）中YY1蛋白水平均升高（分别为48%和19%）。根据YY1蛋白水平进行分层后，YY1阳性表达可识别出88%的PTC患者。YY1表达与PTC和FTC临床病理特征之间的关联表明，YY1表达与诊断时的年龄有关。我们的数据首次表明YY1在分化型甲状腺癌中过表达，且在PTC亚型中YY1过表达更为常见。
