Reddy V K, Fung S J, Zhuo H, Barnes C D
Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology College of Veterinary Medicine, Washington State University, Pullman 99164-6520
Brain Res. 1989 Jul 3;491(1):144-9. doi: 10.1016/0006-8993(89)90096-6.
Two to three days following injection of horseradish peroxidase (HRP) into the spinal cords of 5 cats, the animals were sacrificed and perfused, and the brainstems removed and sectioned. The sections were then processed for HRP and, immunocytochemically, for tyrosine hydroxylase (TH). The dorsolateral pontine tegmentum was divided into the locus coeruleus, subcoeruleus and Kölliker-Fuse nucleus; the mean percentage of pontospinal neurons containing TH were found to be 85.5 +/- 2.5 (S.E.M.), 79.6 +/- 5.6 and 87.1 +/- 3.1, respectively. The cell diameters of locus coeruleus cells were also measured.
在向5只猫的脊髓注射辣根过氧化物酶（HRP）两到三天后，处死动物并进行灌注，取出脑干并切片。然后对切片进行HRP处理，并进行酪氨酸羟化酶（TH）的免疫细胞化学处理。脑桥背外侧被盖分为蓝斑、蓝斑下核和 Kölliker-Fuse 核；发现含有TH的脑桥脊髓神经元的平均百分比分别为85.5±2.5（标准误）、79.6±5.6和87.1±3.1。还测量了蓝斑细胞的细胞直径。
