Yi Jiang, Wu Liguo, Liu Zhou, Zou Haibo, Li Ning, Chen Heping, Liu Jinheng, Li Tao, Zhang Gang
Hepatogastroenterology. 2014 Nov-Dec;61(136):2336-9.
BACKGROUND/AIMS: To evaluate the effect of high-intensity ultrasound (HIFU) ablation on human hepatocellular carcinoma tissues and apoptotic proteins (bcl-2 and p-53).
Patients with hepatocellular carcinoma at stage B were treated with HIFU ablation. Levels of bcl-2 and p53 protein and the apoptosis rate were evaluated both in the pre-treatment and post-treatment tissue specimens using immunochemistry and TUNEL methods, respectively.
After HIFU ablation, p53 protein levels were significantly increased around the coagulation necrosis area, whereas, the level of bcl-2 was significantly decreased. More apoptosis cells were found post ablation compared with those in the pretreatment tissues. Additionally, no significant correlation was found between p53/bcl-2 levels and apoptotic index.
HIFU ablation may exert promote the apoptosis of hepatocellular carcinoma cells and the effect has a closely association with the change of p53 and bcl-2 expression.
背景/目的：评估高强度聚焦超声（HIFU）消融对人肝细胞癌组织及凋亡蛋白（bcl-2和p-53）的影响。
对B期肝细胞癌患者采用HIFU消融治疗。分别使用免疫化学和TUNEL方法，在治疗前和治疗后的组织标本中评估bcl-2和p53蛋白水平以及凋亡率。
HIFU消融后，凝固性坏死区域周围p53蛋白水平显著升高，而bcl-2水平显著降低。与治疗前组织相比，消融后发现更多凋亡细胞。此外，p53/bcl-2水平与凋亡指数之间未发现显著相关性。
HIFU消融可能促进人肝癌细胞凋亡，且该效应与p53和bcl-2表达的变化密切相关。
