Siwicki J K
Department of Immunology, Marie Skłodowska-Curie Memorial Cancer Center, Warsaw, Poland.
Cell Immunol. 1989 Sep;122(2):563-8. doi: 10.1016/0008-8749(89)90102-0.
Human wheat germ agglutinin (WGA)-activated T-lymphocytes from either peripheral blood or spleen could be propagated for several weeks in the presence of culture supernatants from human mononuclear cells obtained after a 1-day stimulation with WGA and after a subsequent 3-day culture without mitogen. The continuous T-cell growth in this culture system required alternate exposition of the cells to the above supernatants, both with and without WGA, but proceeded in the absence of feeder cells. After 3 weeks of propagation most cells displayed the CD4+ phenotype, expressed IL2 receptors, and responded to PHA, Con A, and WGA. It has been shown that WGA-activated T-cells could be cloned by limiting dilution and propagated using the above culture system.
人麦胚凝集素（WGA）激活的外周血或脾脏T淋巴细胞，在来自人单核细胞的培养上清液存在的情况下，可以增殖数周。这些人单核细胞先用WGA刺激1天，随后在无丝裂原的情况下培养3天。在该培养系统中T细胞的持续生长需要细胞交替暴露于上述有WGA和无WGA的上清液中，但在没有饲养细胞的情况下也能进行。增殖3周后，大多数细胞呈现CD4 +表型，表达IL2受体，并对PHA、Con A和WGA有反应。已经表明，WGA激活的T细胞可以通过有限稀释进行克隆，并使用上述培养系统进行增殖。
