Kelwick Richard, Kopniczky Margarita, Bower Iain, Chi Wenqiang, Chin Matthew Ho Wai, Fan Sisi, Pilcher Jemma, Strutt James, Webb Alexander J, Jensen Kirsten, Stan Guy-Bart, Kitney Richard, Freemont Paul
Centre for Synthetic Biology and Innovation, South Kensington Campus, London, United Kingdom; Department of Medicine, South Kensington Campus, London, United Kingdom.
Centre for Synthetic Biology and Innovation, South Kensington Campus, London, United Kingdom; Department of Life Sciences, South Kensington Campus, London, United Kingdom.
PLoS One. 2015 Feb 20;10(2):e0117202. doi: 10.1371/journal.pone.0117202. eCollection 2015.
Biopolymers, such as poly-3-hydroxybutyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility. Despite these advantages, P(3HB) production is currently more expensive than the production of oil-derived plastics, and therefore, more efficient P(3HB) production processes would be desirable. In this study, we describe the model-guided design and experimental validation of several engineered P(3HB) producing operons. In particular, we describe the characterization of a hybrid phaCAB operon that consists of a dual promoter (native and J23104) and RBS (native and B0034) design. P(3HB) production at 24 h was around six-fold higher in hybrid phaCAB engineered Escherichia coli in comparison to E. coli engineered with the native phaCAB operon from Ralstonia eutropha H16. Additionally, we describe the utilization of non-recyclable waste as a low-cost carbon source for the production of P(3HB).
生物聚合物，如聚-3-羟基丁酸酯（P(3HB)），作为一种碳储存物质在一系列生物体中产生，并且具有与石油衍生塑料相似的特性，但还具有生物可降解性和生物相容性等额外优势。尽管有这些优点，但目前P(3HB)的生产成本高于石油衍生塑料，因此，更高效的P(3HB)生产工艺是可取的。在本研究中，我们描述了几种工程化P(3HB)生产操纵子的模型引导设计和实验验证。特别是，我们描述了一种由双启动子（天然启动子和J23104）和核糖体结合位点（天然RBS和B0034）设计组成的杂交phaCAB操纵子的特性。与用来自嗜油产碱杆菌H16的天然phaCAB操纵子工程化的大肠杆菌相比，杂交phaCAB工程化的大肠杆菌在24小时时的P(3HB)产量高出约六倍。此外，我们描述了利用不可回收废物作为生产P(3HB)的低成本碳源。
