Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstraße 3, D-48149, Münster, Germany.
AMB Express. 2012 Nov 19;2(1):59. doi: 10.1186/2191-0855-2-59.
Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery of a pure product at low costs. This study presents a simplified method for large scale poly(3-hydroxybutyrate), poly(3HB), extraction using sodium hypochlorite. Poly(3HB) was extracted from cells of Ralstonia eutropha H16 at almost 96% purity. At different extraction volumes, a maximum recovery rate of 91.32% was obtained. At the largest extraction volume of 50 L, poly(3HB) with an average purity of 93.32% ± 4.62% was extracted with a maximum recovery of 87.03% of the initial poly(3HB) content. This process is easy to handle and requires less efforts than previously described processes.
从细菌细胞物质中分离聚羟基烷酸（PHA）是实现聚合物盈利生产的关键步骤。因此，提取方法必须以低成本实现高纯度产物的高回收率。本研究提出了一种使用次氯酸钠从罗尔斯顿氏菌属（Ralstonia eutropha）H16 中大规模提取聚（3-羟基丁酸酯）（poly(3HB)）的简化方法。聚（3HB）几乎以 96%的纯度从细胞中提取出来。在不同的提取体积下，获得了 91.32%的最大回收率。在最大提取体积为 50 L 的情况下，提取了平均纯度为 93.32%±4.62%的聚（3HB），初始聚（3HB）含量的最大回收率为 87.03%。该过程易于操作，所需工作量比以前描述的过程少。
