一种高通量金纳米颗粒化学发光法检测阿片受体拮抗剂盐酸纳洛酮。
A high throughput gold nanoparticles chemiluminescence detection of opioid receptor antagonist naloxone hydrochloride.
作者信息
Alarfaj Nawal A, El-Tohamy Maha F
机构信息
Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh, 11495 Saudi Arabia.
Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh, 11495 Saudi Arabia ; Permanent address: General Administrative of Medical Affairs, Zagazig University, Zagazig, Egypt.
出版信息
Chem Cent J. 2015 Feb 11;9:6. doi: 10.1186/s13065-015-0083-6. eCollection 2015.
BACKGROUND
The opioid antagonist agent naloxone hydrochloride (NLX) is a drug that has high affinity for opiate receptors but do not activate these receptors. Owing to the role of this drug to block the effects of exogenous administered opioids and endogenous released endorphians we can deduce the importance of developing sensitive analytical methods for detection of such drug. In the present study gold nanoparticles (AuNPs) was employed for enhancing the chemiluminescence (CL) signals arising from luminol-ferricyanide reaction in the presence of naloxone hydrochloride using sequential injection chemiluminescence analysis (SIA).
METHOD
In the present study gold nanoparticles (AuNPs) was employed for enhancing the chemiluminescence (CL) signals arising from luminol-ferricyanide reaction in the presence of naloxone hydrochloride using sequential injection chemiluminescence analysis (SIA).
RESULTS
The developed method was examined under optimum experimental conditions and the obtained results revealed a linear relationship between the relative CL intensity and the investigated drug at a concentration range of 1.0×10(-9)-1.0×10(-2) mol L(-1), (r = 0.9993, n=9) with detection and quantification limits of 1.6×10(-11) and 1.0×10(-9) mol L(-1), respectively. The relative standard deviation was 0.9%.
CONCLUSION
The proposed method was employed for the determination of the investigated drug in bulk powder, its pharmaceutical dosage forms and biological fluids. The interference of some metals and amino acids on the CL intensity was investigated. Also the interference of some related pharmacological action drugs was tested. The obtained results of the developed method were statistically treated and compared with those obtained from other reported methods. Graphical AbstractUtility of gold nanparticles in luminol-potassium ferricyanide chemiluminescence system for determination of naloxone hydrochloride.
背景
阿片类拮抗剂盐酸纳洛酮（NLX）是一种对阿片受体具有高亲和力但不激活这些受体的药物。由于该药物具有阻断外源性给予的阿片类药物和内源性释放的内啡肽作用，我们可以推断出开发用于检测此类药物的灵敏分析方法的重要性。在本研究中，使用顺序注射化学发光分析（SIA），采用金纳米粒子（AuNPs）增强在盐酸纳洛酮存在下鲁米诺 - 铁氰化物反应产生的化学发光（CL）信号。
方法
在本研究中，使用顺序注射化学发光分析（SIA），采用金纳米粒子（AuNPs）增强在盐酸纳洛酮存在下鲁米诺 - 铁氰化物反应产生的化学发光（CL）信号。
结果
在最佳实验条件下对所开发的方法进行了研究，所得结果表明在1.0×10⁻⁹ - 1.0×10⁻² mol L⁻¹的浓度范围内，相对CL强度与被测药物之间存在线性关系，（r = 0.9993，n = 9），检测限和定量限分别为1.6×10⁻¹¹和1.0×10⁻⁹ mol L⁻¹。相对标准偏差为0.9%。
结论
所提出的方法用于测定原料药粉、其药物剂型和生物流体中的被测药物。研究了一些金属和氨基酸对CL强度的干扰。还测试了一些相关药理作用药物的干扰。对所开发方法获得的结果进行了统计学处理，并与其他报道方法获得的结果进行了比较。图形摘要金纳米粒子在鲁米诺 - 铁氰化钾化学发光体系中用于测定盐酸纳洛酮的应用。
Zotero 插件