Vyšniauskas Aurimas, Balaz Milan, Anderson Harry L, Kuimova Marina K
Chemistry Department, Imperial College London, Exhibition Road, SW7 2AZ, UK.
Phys Chem Chem Phys. 2015 Mar 21;17(11):7548-54. doi: 10.1039/c5cp00439j.
Microviscosity is of paramount importance in materials and bio-sciences. Fluorescence imaging using molecular rotors has emerged as a versatile tool to measure microviscosity, either using a fluorescence lifetime or a ratiometric signal of the rotor; however, only a limited number of blue-to-green-emitting fluorophores with both the lifetime and the ratiometric signal sensitivity to viscosity have been reported to date. Here we report a deep red emitting dual viscosity sensor, which allows both the ratiometric and the lifetime imaging of viscosity. We study viscosity in a range of lipid-based systems and conclude that in complex dynamic systems dual detection is preferable in order to independently verify the results of the measurements as well as perform rapid detection of changing viscosity.
微观粘度在材料科学和生物科学中至关重要。使用分子转子的荧光成像已成为一种通用工具，可通过荧光寿命或转子的比率信号来测量微观粘度；然而，迄今为止，仅报道了少数对粘度具有寿命和比率信号敏感性的蓝到绿发射荧光团。在此，我们报道了一种深红色发射的双粘度传感器，它能够实现粘度的比率成像和寿命成像。我们研究了一系列基于脂质的系统中的粘度，并得出结论，在复杂的动态系统中，双重检测更为可取，以便独立验证测量结果并快速检测变化的粘度。
