Vyšniauskas Aurimas, Qurashi Maryam, Gallop Nathaniel, Balaz Milan, Anderson Harry L, Kuimova Marina K
Chemistry Department , Imperial College London , Exhibition Road , SW7 2AZ , UK . Email:
Chemistry Department , University of Oxford , Chemistry Research Laboratory , Oxford , OX1 3TA , UK.
Chem Sci. 2015 Oct 1;6(10):5773-5778. doi: 10.1039/c5sc02248g. Epub 2015 Jul 6.
Viscosity and temperature variations in the microscopic world are of paramount importance for diffusion and reactions. Consequently, a plethora of fluorescent probes have evolved over the years to enable fluorescent imaging of both parameters in biological cells. However, the simultaneous effect of both temperature and viscosity on the photophysical behavior of fluorophores is rarely considered, yet unavoidable variations in temperature can lead to significant errors in the readout of viscosity and . Here we examine the effect of temperature on the photophysical behavior of three classes of viscosity-sensitive fluorophores termed 'molecular rotors'. For each of the fluorophores we decouple the effect of temperature from the effect of viscosity. In the case of the conjugated porphyrin dimer, we demonstrate that, uniquely, simultaneous dual-mode lifetime and intensity measurements of this fluorophore can be used for measuring both viscosity and temperature concurrently.
微观世界中的粘度和温度变化对于扩散和反应至关重要。因此，多年来已开发出大量荧光探针，以实现对生物细胞中这两个参数的荧光成像。然而，温度和粘度对荧光团光物理行为的同时影响很少被考虑，而温度的不可避免变化会导致粘度读数出现重大误差。在这里，我们研究了温度对三类称为“分子转子”的粘度敏感荧光团光物理行为的影响。对于每种荧光团，我们将温度的影响与粘度的影响分离开来。在共轭卟啉二聚体的情况下，我们证明，独特的是，该荧光团的同时双模式寿命和强度测量可用于同时测量粘度和温度。
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