Flourakis Matthieu, Allada Ravi
Department of Neurobiology, Northwestern University, Evanston, Illinois, USA.
Methods Enzymol. 2015;552:23-44. doi: 10.1016/bs.mie.2014.10.005. Epub 2014 Dec 26.
Circadian clocks modulate the action potential firing frequency of pacemaker neurons. This daily variation in membrane excitability has been described in multiple species: from mollusks to fruit flies and mammals. Here, we provide an overview of the Drosophila pacemaker neural network, how circadian clocks drive neuronal activity within this network and we will present electrophysiological methods that we have applied to directly measure neuronal activity and reveal signal transduction pathways.
生物钟调节起搏器神经元的动作电位发放频率。这种膜兴奋性的每日变化已在多个物种中得到描述：从软体动物到果蝇和哺乳动物。在这里，我们概述了果蝇起搏器神经网络，生物钟如何驱动该网络内的神经元活动，并且我们将介绍我们已应用于直接测量神经元活动并揭示信号转导途径的电生理方法。
